Low molecular thymic peptides stimulate human blood dendritic cells
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Low molecular thymic peptides stimulate human blood dendritic cells. / Mayer, G; Pohlmeyer, K; Caliebe, A; Heimueller, E; Behnke, B; Steimann, G; Lange, Claudia; Beuth, J.
in: ANTICANCER RES, Jahrgang 20, Nr. 5A, 2000, S. 2873-83.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Low molecular thymic peptides stimulate human blood dendritic cells
AU - Mayer, G
AU - Pohlmeyer, K
AU - Caliebe, A
AU - Heimueller, E
AU - Behnke, B
AU - Steimann, G
AU - Lange, Claudia
AU - Beuth, J
PY - 2000
Y1 - 2000
N2 - Dendritic cells are considered to be the most potent antigen-presenting cells and are thus promising new tools for the immunotherapy of cancer. They respond to various stimuli by differentiation (expression of CD83) and up-regulation of costimulatory surface molecules. Thymic peptides have immunostimulatory and immunomodulating properties. Their therapeutic potential in immunotherapy of cancer has been discussed. To test whether thymic peptides act on dendritic cells, we examined the effects of a standardized thymic peptide preparation on cultured human monocyte-derived dendritic cells. Addition of thymic peptides resulted in enhanced expression of the specific differentiation marker CD83 in a dose dependent manner. Moreover, thymic peptides induced the up-regulation of costimulatory molecules including CD86, CD80, HLA-DR and HLA-ABC. After priming with thymic peptides dendritic cells showed an enhanced expression of IL-8 and TNF-alpha mRNA and protein release. Dendritic cells stimulated with thymic peptides were able to induce proliferation of autologous T cells as measured by 3H-thymidine incorporation in mixed Lymphocyte reaction. In combination with a low dosage of keyhole limpet hemocyanin, thymic peptides showed additive effects in the up-regulation of CD83 and costimulatory surface markers. Our findings indicate that thymic peptides per se act on professional antigen-presenting cells in a stimulatory manner and were presented by these cells. Furthermore, thymic peptides enhance the response of dendritic cells to low dosages of a standard nominal antigen. Therefore, thymic peptides could improve the immunological activity especially against low amounts of endogenous antigens.
AB - Dendritic cells are considered to be the most potent antigen-presenting cells and are thus promising new tools for the immunotherapy of cancer. They respond to various stimuli by differentiation (expression of CD83) and up-regulation of costimulatory surface molecules. Thymic peptides have immunostimulatory and immunomodulating properties. Their therapeutic potential in immunotherapy of cancer has been discussed. To test whether thymic peptides act on dendritic cells, we examined the effects of a standardized thymic peptide preparation on cultured human monocyte-derived dendritic cells. Addition of thymic peptides resulted in enhanced expression of the specific differentiation marker CD83 in a dose dependent manner. Moreover, thymic peptides induced the up-regulation of costimulatory molecules including CD86, CD80, HLA-DR and HLA-ABC. After priming with thymic peptides dendritic cells showed an enhanced expression of IL-8 and TNF-alpha mRNA and protein release. Dendritic cells stimulated with thymic peptides were able to induce proliferation of autologous T cells as measured by 3H-thymidine incorporation in mixed Lymphocyte reaction. In combination with a low dosage of keyhole limpet hemocyanin, thymic peptides showed additive effects in the up-regulation of CD83 and costimulatory surface markers. Our findings indicate that thymic peptides per se act on professional antigen-presenting cells in a stimulatory manner and were presented by these cells. Furthermore, thymic peptides enhance the response of dendritic cells to low dosages of a standard nominal antigen. Therefore, thymic peptides could improve the immunological activity especially against low amounts of endogenous antigens.
KW - Amino Acid Sequence
KW - Animals
KW - Antigens, CD
KW - Antigens, CD40
KW - Antigens, CD80
KW - Antigens, CD86
KW - Cattle
KW - Cell Differentiation
KW - Cell Division
KW - Cells, Cultured
KW - Dendritic Cells
KW - Gene Expression Regulation
KW - HLA-DR Antigens
KW - Haptens
KW - Hemocyanin
KW - Humans
KW - Interleukin-8
KW - Membrane Glycoproteins
KW - Molecular Sequence Data
KW - Molecular Weight
KW - Peptides
KW - RNA, Messenger
KW - T-Lymphocytes
KW - Thymus Gland
KW - Tumor Necrosis Factor-alpha
KW - Up-Regulation
M3 - SCORING: Journal article
C2 - 11062696
VL - 20
SP - 2873
EP - 2883
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 5A
ER -