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Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice

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Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice. / Schumacher, U; van Damme, E J; Peumans, W J; Butterfield, J H; Mitchell, B S.

In: ACTA HISTOCHEM, Vol. 100, No. 1, 02.1998, p. 1-9.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schumacher, U, van Damme, EJ, Peumans, WJ, Butterfield, JH & Mitchell, BS 1998, 'Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice', ACTA HISTOCHEM, vol. 100, no. 1, pp. 1-9. https://doi.org/10.1016/S0065-1281(98)80002-0

APA

Schumacher, U., van Damme, E. J., Peumans, W. J., Butterfield, J. H., & Mitchell, B. S. (1998). Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice. ACTA HISTOCHEM, 100(1), 1-9. https://doi.org/10.1016/S0065-1281(98)80002-0

Vancouver

Schumacher U, van Damme EJ, Peumans WJ, Butterfield JH, Mitchell BS. Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice. ACTA HISTOCHEM. 1998 Feb;100(1):1-9. https://doi.org/10.1016/S0065-1281(98)80002-0

Bibtex

@article{f6f7c3c3b67d45eaaadf22d70a335567,
title = "Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice",
abstract = "It is difficult to isolate and impossible to propagate human mast cells in tissue culture. As an alternative to the use of human differentiated mast cells, a human leukaemic mast cell line (HMC-1), which can be propagated in vitro, has been employed in a number of studies. Carbohydrate binding proteins, lectins, have been used to characterise the terminal sugar residues of human mast cells in situ. The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice. Lectins specific for the complex carbohydrates, neuraminic acid and N-acetylglucosamine residues showed generally a strong uniform binding pattern, whereas mannose and glucose specific yielded lectins a greater heterogeneity. This glycotope expression pattern has some similarities with those of human mast cells in situ, and therefore HMC-1 cells grown in scid mice constitute a valuable model system for the study of carbohydrate expression in human mast cells.",
keywords = "Animals, Disease Models, Animal, Female, Histocytochemistry, Humans, Injections, Intravenous, Injections, Subcutaneous, Lectins, Leukemia, Mast-Cell, Male, Mice, Mice, Inbred BALB C, Mice, SCID, Neoplasm Transplantation, Tumor Cells, Cultured",
author = "U Schumacher and {van Damme}, {E J} and Peumans, {W J} and Butterfield, {J H} and Mitchell, {B S}",
year = "1998",
month = feb,
doi = "10.1016/S0065-1281(98)80002-0",
language = "English",
volume = "100",
pages = "1--9",
journal = "ACTA HISTOCHEM",
issn = "0065-1281",
publisher = "Urban und Fischer Verlag Jena",
number = "1",

}

RIS

TY - JOUR

T1 - Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice

AU - Schumacher, U

AU - van Damme, E J

AU - Peumans, W J

AU - Butterfield, J H

AU - Mitchell, B S

PY - 1998/2

Y1 - 1998/2

N2 - It is difficult to isolate and impossible to propagate human mast cells in tissue culture. As an alternative to the use of human differentiated mast cells, a human leukaemic mast cell line (HMC-1), which can be propagated in vitro, has been employed in a number of studies. Carbohydrate binding proteins, lectins, have been used to characterise the terminal sugar residues of human mast cells in situ. The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice. Lectins specific for the complex carbohydrates, neuraminic acid and N-acetylglucosamine residues showed generally a strong uniform binding pattern, whereas mannose and glucose specific yielded lectins a greater heterogeneity. This glycotope expression pattern has some similarities with those of human mast cells in situ, and therefore HMC-1 cells grown in scid mice constitute a valuable model system for the study of carbohydrate expression in human mast cells.

AB - It is difficult to isolate and impossible to propagate human mast cells in tissue culture. As an alternative to the use of human differentiated mast cells, a human leukaemic mast cell line (HMC-1), which can be propagated in vitro, has been employed in a number of studies. Carbohydrate binding proteins, lectins, have been used to characterise the terminal sugar residues of human mast cells in situ. The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice. Lectins specific for the complex carbohydrates, neuraminic acid and N-acetylglucosamine residues showed generally a strong uniform binding pattern, whereas mannose and glucose specific yielded lectins a greater heterogeneity. This glycotope expression pattern has some similarities with those of human mast cells in situ, and therefore HMC-1 cells grown in scid mice constitute a valuable model system for the study of carbohydrate expression in human mast cells.

KW - Animals

KW - Disease Models, Animal

KW - Female

KW - Histocytochemistry

KW - Humans

KW - Injections, Intravenous

KW - Injections, Subcutaneous

KW - Lectins

KW - Leukemia, Mast-Cell

KW - Male

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, SCID

KW - Neoplasm Transplantation

KW - Tumor Cells, Cultured

U2 - 10.1016/S0065-1281(98)80002-0

DO - 10.1016/S0065-1281(98)80002-0

M3 - SCORING: Journal article

C2 - 9542577

VL - 100

SP - 1

EP - 9

JO - ACTA HISTOCHEM

JF - ACTA HISTOCHEM

SN - 0065-1281

IS - 1

ER -