Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice
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Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice. / Schumacher, U; van Damme, E J; Peumans, W J; Butterfield, J H; Mitchell, B S.
in: ACTA HISTOCHEM, Jahrgang 100, Nr. 1, 02.1998, S. 1-9.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Lectin histochemistry of human leukaemic mast cells (HMC-1) transplanted into severe combined immunodeficient (scid) mice
AU - Schumacher, U
AU - van Damme, E J
AU - Peumans, W J
AU - Butterfield, J H
AU - Mitchell, B S
PY - 1998/2
Y1 - 1998/2
N2 - It is difficult to isolate and impossible to propagate human mast cells in tissue culture. As an alternative to the use of human differentiated mast cells, a human leukaemic mast cell line (HMC-1), which can be propagated in vitro, has been employed in a number of studies. Carbohydrate binding proteins, lectins, have been used to characterise the terminal sugar residues of human mast cells in situ. The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice. Lectins specific for the complex carbohydrates, neuraminic acid and N-acetylglucosamine residues showed generally a strong uniform binding pattern, whereas mannose and glucose specific yielded lectins a greater heterogeneity. This glycotope expression pattern has some similarities with those of human mast cells in situ, and therefore HMC-1 cells grown in scid mice constitute a valuable model system for the study of carbohydrate expression in human mast cells.
AB - It is difficult to isolate and impossible to propagate human mast cells in tissue culture. As an alternative to the use of human differentiated mast cells, a human leukaemic mast cell line (HMC-1), which can be propagated in vitro, has been employed in a number of studies. Carbohydrate binding proteins, lectins, have been used to characterise the terminal sugar residues of human mast cells in situ. The aim of the present study is to characterise the lectin binding sites of HMC-1 cells transplanted into severe combined immunodeficient (scid) mice. Lectins specific for the complex carbohydrates, neuraminic acid and N-acetylglucosamine residues showed generally a strong uniform binding pattern, whereas mannose and glucose specific yielded lectins a greater heterogeneity. This glycotope expression pattern has some similarities with those of human mast cells in situ, and therefore HMC-1 cells grown in scid mice constitute a valuable model system for the study of carbohydrate expression in human mast cells.
KW - Animals
KW - Disease Models, Animal
KW - Female
KW - Histocytochemistry
KW - Humans
KW - Injections, Intravenous
KW - Injections, Subcutaneous
KW - Lectins
KW - Leukemia, Mast-Cell
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Mice, SCID
KW - Neoplasm Transplantation
KW - Tumor Cells, Cultured
U2 - 10.1016/S0065-1281(98)80002-0
DO - 10.1016/S0065-1281(98)80002-0
M3 - SCORING: Journal article
C2 - 9542577
VL - 100
SP - 1
EP - 9
JO - ACTA HISTOCHEM
JF - ACTA HISTOCHEM
SN - 0065-1281
IS - 1
ER -