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Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells

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Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells. / Gordon, P R; Leimig, T; Babarin-Dorner, A; Houston, J; Holladay, M; Mueller, I; Geiger, T; Handgretinger, R.

In: BONE MARROW TRANSPL, Vol. 31, No. 1, 01.2003, p. 17-22.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gordon, PR, Leimig, T, Babarin-Dorner, A, Houston, J, Holladay, M, Mueller, I, Geiger, T & Handgretinger, R 2003, 'Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells', BONE MARROW TRANSPL, vol. 31, no. 1, pp. 17-22. https://doi.org/10.1038/sj.bmt.1703792

APA

Gordon, P. R., Leimig, T., Babarin-Dorner, A., Houston, J., Holladay, M., Mueller, I., Geiger, T., & Handgretinger, R. (2003). Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells. BONE MARROW TRANSPL, 31(1), 17-22. https://doi.org/10.1038/sj.bmt.1703792

Vancouver

Gordon PR, Leimig T, Babarin-Dorner A, Houston J, Holladay M, Mueller I et al. Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells. BONE MARROW TRANSPL. 2003 Jan;31(1):17-22. https://doi.org/10.1038/sj.bmt.1703792

Bibtex

@article{1b940c7a1bef400fb67c62d71b6749c4,
title = "Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells",
abstract = "We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.",
keywords = "Adult, Animals, Antigens, CD, Cell Separation, Colony-Forming Units Assay, DNA Primers, Filgrastim, Flow Cytometry, Glycoproteins, Granulocyte Colony-Stimulating Factor, Hematopoietic Stem Cell Mobilization, Hematopoietic Stem Cells, Humans, Leukapheresis, Living Donors, Mice, Mice, Inbred NOD, Mice, SCID, Peptides, Polymerase Chain Reaction, Recombinant Proteins, Transplantation, Heterologous",
author = "Gordon, {P R} and T Leimig and A Babarin-Dorner and J Houston and M Holladay and I Mueller and T Geiger and R Handgretinger",
year = "2003",
month = jan,
doi = "10.1038/sj.bmt.1703792",
language = "English",
volume = "31",
pages = "17--22",
journal = "BONE MARROW TRANSPL",
issn = "0268-3369",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells

AU - Gordon, P R

AU - Leimig, T

AU - Babarin-Dorner, A

AU - Houston, J

AU - Holladay, M

AU - Mueller, I

AU - Geiger, T

AU - Handgretinger, R

PY - 2003/1

Y1 - 2003/1

N2 - We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.

AB - We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.

KW - Adult

KW - Animals

KW - Antigens, CD

KW - Cell Separation

KW - Colony-Forming Units Assay

KW - DNA Primers

KW - Filgrastim

KW - Flow Cytometry

KW - Glycoproteins

KW - Granulocyte Colony-Stimulating Factor

KW - Hematopoietic Stem Cell Mobilization

KW - Hematopoietic Stem Cells

KW - Humans

KW - Leukapheresis

KW - Living Donors

KW - Mice

KW - Mice, Inbred NOD

KW - Mice, SCID

KW - Peptides

KW - Polymerase Chain Reaction

KW - Recombinant Proteins

KW - Transplantation, Heterologous

U2 - 10.1038/sj.bmt.1703792

DO - 10.1038/sj.bmt.1703792

M3 - SCORING: Journal article

C2 - 12621502

VL - 31

SP - 17

EP - 22

JO - BONE MARROW TRANSPL

JF - BONE MARROW TRANSPL

SN - 0268-3369

IS - 1

ER -