Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells
Standard
Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells. / Gordon, P R; Leimig, T; Babarin-Dorner, A; Houston, J; Holladay, M; Mueller, I; Geiger, T; Handgretinger, R.
in: BONE MARROW TRANSPL, Jahrgang 31, Nr. 1, 01.2003, S. 17-22.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells
AU - Gordon, P R
AU - Leimig, T
AU - Babarin-Dorner, A
AU - Houston, J
AU - Holladay, M
AU - Mueller, I
AU - Geiger, T
AU - Handgretinger, R
PY - 2003/1
Y1 - 2003/1
N2 - We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.
AB - We have evaluated the feasibility of large-scale isolation of CD133+ progenitors from healthy mobilized adult donors for potential clinical use in autologous and allogeneic transplantation. A total of 11 healthy volunteer adult donors were mobilized with G-CSF. CD133+ stem cells were isolated from a single leukapheresis using the Clinimacs method. The median percentage of CD133 before positive selection was 0.75% (range 0.39-2.03%). After selection, the median purity and recovery was 94% (range 85.2-98.0%) and 69% (range 44-100%), respectively. The median log10 T-cell depletion obtained by CD133+ positive selection was 4.2 (range 3.8-4.7). The CD133+ progenitors were highly enriched in colony-forming units (CFU) and transplantation into NOD/SCID mice resulted in a high engraftment rate. Transplantation of sorted CD133+/CD34+ cells into NOD/SCID mice showed a higher engraftment compared to CD133-/CD34+ cells. Mobilized peripheral CD133+ stem cells can be purified in large scale for potential clinical use. The biological function of the cells is not impaired. The majority of the NOD/SCID repopulating cells are within the CD133+/CD34+ subpopulation. Therefore, clinical studies using purified CD133+ stem cells can be envisoned to further clarify the role of CD133+ stem cells in hematopoietic reconstitution after transplantation.
KW - Adult
KW - Animals
KW - Antigens, CD
KW - Cell Separation
KW - Colony-Forming Units Assay
KW - DNA Primers
KW - Filgrastim
KW - Flow Cytometry
KW - Glycoproteins
KW - Granulocyte Colony-Stimulating Factor
KW - Hematopoietic Stem Cell Mobilization
KW - Hematopoietic Stem Cells
KW - Humans
KW - Leukapheresis
KW - Living Donors
KW - Mice
KW - Mice, Inbred NOD
KW - Mice, SCID
KW - Peptides
KW - Polymerase Chain Reaction
KW - Recombinant Proteins
KW - Transplantation, Heterologous
U2 - 10.1038/sj.bmt.1703792
DO - 10.1038/sj.bmt.1703792
M3 - SCORING: Journal article
C2 - 12621502
VL - 31
SP - 17
EP - 22
JO - BONE MARROW TRANSPL
JF - BONE MARROW TRANSPL
SN - 0268-3369
IS - 1
ER -