Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease
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Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease. / Barreiro, Karina; Huber, Tobias B; Holthofer, Harry.
In: Methods Mol Biol, Vol. 2067, 2020, p. 175-188.Research output: SCORING: Contribution to journal › SCORING: Review article › Research
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TY - JOUR
T1 - Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease
AU - Barreiro, Karina
AU - Huber, Tobias B
AU - Holthofer, Harry
PY - 2020
Y1 - 2020
N2 - Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.
AB - Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.
KW - Diabetic Nephropathies/diagnosis
KW - Dialysis/instrumentation
KW - Extracellular Vesicles/pathology
KW - Feasibility Studies
KW - Humans
KW - Kidney Function Tests/instrumentation
KW - Kidney/pathology
KW - Urinalysis/instrumentation
U2 - 10.1007/978-1-4939-9841-8_13
DO - 10.1007/978-1-4939-9841-8_13
M3 - SCORING: Review article
C2 - 31701453
VL - 2067
SP - 175
EP - 188
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -