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Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease

Standard

Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease. / Barreiro, Karina; Huber, Tobias B; Holthofer, Harry.

in: Methods Mol Biol, Jahrgang 2067, 2020, S. 175-188.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung

Harvard

Barreiro, K, Huber, TB & Holthofer, H 2020, 'Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease', Methods Mol Biol, Jg. 2067, S. 175-188. https://doi.org/10.1007/978-1-4939-9841-8_13

APA

Barreiro, K., Huber, T. B., & Holthofer, H. (2020). Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease. Methods Mol Biol, 2067, 175-188. https://doi.org/10.1007/978-1-4939-9841-8_13

Vancouver

Barreiro K, Huber TB, Holthofer H. Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease. Methods Mol Biol. 2020;2067:175-188. https://doi.org/10.1007/978-1-4939-9841-8_13

Bibtex

@article{fb29d9e305bb4f689095b233fb04975e,
title = "Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease",
abstract = "Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.",
keywords = "Diabetic Nephropathies/diagnosis, Dialysis/instrumentation, Extracellular Vesicles/pathology, Feasibility Studies, Humans, Kidney Function Tests/instrumentation, Kidney/pathology, Urinalysis/instrumentation",
author = "Karina Barreiro and Huber, {Tobias B} and Harry Holthofer",
year = "2020",
doi = "10.1007/978-1-4939-9841-8_13",
language = "English",
volume = "2067",
pages = "175--188",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease

AU - Barreiro, Karina

AU - Huber, Tobias B

AU - Holthofer, Harry

PY - 2020

Y1 - 2020

N2 - Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.

AB - Extracellular vesicles are lipid bilayer enclosed structures secreted by all cell types. Their cargo includes proteins, lipids, RNAs, and DNA, which reflect the physiological state of their cells of origin. Recently, urinary extracellular vesicles have emerged as a valuable source of biomarkers for kidney and systemic disease.Unfortunately, all existing methods for extracellular vesicle isolation from urine are time consuming and/or expensive. Thus, they are not adaptable to large-scale studies and unsuitable for clinical use without special equipment in the laboratory. Recently, our group has devised a set of new, quick, simple, and inexpensive techniques, based on hydrostatic filtration dialysis (HFD) of urine extremely suitable for diagnostic purposes. This novel approach represents a great potential for new diagnostics and understanding disease biology in general and brings the biomarker detection to the scope of all laboratories.

KW - Diabetic Nephropathies/diagnosis

KW - Dialysis/instrumentation

KW - Extracellular Vesicles/pathology

KW - Feasibility Studies

KW - Humans

KW - Kidney Function Tests/instrumentation

KW - Kidney/pathology

KW - Urinalysis/instrumentation

U2 - 10.1007/978-1-4939-9841-8_13

DO - 10.1007/978-1-4939-9841-8_13

M3 - SCORING: Review article

C2 - 31701453

VL - 2067

SP - 175

EP - 188

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -