Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma?
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Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma? / Braga, Walter Mt; da Silva, Bruna R; Alves, Veruska Lf; Bortoluzo, Adriana B; Atanackovic, Djordje; Colleoni, Gisele Wb.
In: IMMUNOTHERAPY-UK, Vol. 6, No. 5, 2014, p. 569-75.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Is there any relationship between gene expression of tumor antigens and CD4+ T cells in multiple myeloma?
AU - Braga, Walter Mt
AU - da Silva, Bruna R
AU - Alves, Veruska Lf
AU - Bortoluzo, Adriana B
AU - Atanackovic, Djordje
AU - Colleoni, Gisele Wb
PY - 2014
Y1 - 2014
N2 - AIM: The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor-infiltrating T cells.MATERIALS & METHODS: MAGE-C1/CT-7, MAGEA3/6, NY-ESO-1, LAGE-1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM) aspirates by RT-PCR. Expression of FOXP3/CTLA4 and RORyt, as markers for Tregs and Th17 cells, respectively, was investigated by quantitative PCR.RESULTS: MAGEC1/CT7 was expressed in 66% of MM samples. We did not find correlation between the presence of single CTA and expression of CTLA4 or RORyt neither expression of CD4(+) T-cell markers and the number of CTA simultaneously expressed in the tumor. However, we did observe a correlation between the percentage of plasma cells and the number of CTAs expressed in the patients' bone marrow.CONCLUSION: Although CTAs and immunomodulatory CD4(+) T cells represent potential targets for immunotherapy in MM, we did not find association among expression of such genes in MM.
AB - AIM: The present study aimed at correlating the expression of cancer/testis antigens (CTAs) with the expression of genes related to tumor-infiltrating T cells.MATERIALS & METHODS: MAGE-C1/CT-7, MAGEA3/6, NY-ESO-1, LAGE-1 and GAGE expression were evaluated in 46 bone marrow multiple myeloma (MM) aspirates by RT-PCR. Expression of FOXP3/CTLA4 and RORyt, as markers for Tregs and Th17 cells, respectively, was investigated by quantitative PCR.RESULTS: MAGEC1/CT7 was expressed in 66% of MM samples. We did not find correlation between the presence of single CTA and expression of CTLA4 or RORyt neither expression of CD4(+) T-cell markers and the number of CTA simultaneously expressed in the tumor. However, we did observe a correlation between the percentage of plasma cells and the number of CTAs expressed in the patients' bone marrow.CONCLUSION: Although CTAs and immunomodulatory CD4(+) T cells represent potential targets for immunotherapy in MM, we did not find association among expression of such genes in MM.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antigens, Neoplasm
KW - Bone Marrow
KW - CD4-Positive T-Lymphocytes
KW - Female
KW - Gene Expression Regulation, Neoplastic
KW - Humans
KW - Male
KW - Middle Aged
KW - Multiple Myeloma
KW - Neoplasm Proteins
KW - Plasma Cells
KW - Tumor Markers, Biological
U2 - 10.2217/imt.14.24
DO - 10.2217/imt.14.24
M3 - SCORING: Journal article
C2 - 24896625
VL - 6
SP - 569
EP - 575
JO - IMMUNOTHERAPY-UK
JF - IMMUNOTHERAPY-UK
SN - 1750-743X
IS - 5
ER -