International study on inter-reader variability for circulating tumor cells in breast cancer
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International study on inter-reader variability for circulating tumor cells in breast cancer. / Ignatiadis, Michail; Riethdorf, Sabine; Bidard, François-Clement; Vaucher, Isabelle; Khazour, Mustapha; Rothé, Françoise; Metallo, Jessica; Rouas, Ghizlane; Payne, Rachel E; Coombes, Raoul; Teufel, Ingrid; Andergassen, Ulrich; Apostolaki, Stella; Politaki, Eleni; Mavroudis, Dimitris; Bessi, Silvia; Pestrin, Marta; Di Leo, Angelo; Campion, Michael; Reinholz, Monica; Perez, Edith; Piccart, Martine; Borgen, Elin; Naume, Bjorn; Jimenez, Jose; Aura, Claudia; Zorzino, Laura; Cassatella, Maria; Sandri, Maria; Mostert, Bianca; Sleijfer, Stefan; Kraan, Jaco; Janni, Wolfgang; Fehm, Tanja; Rack, Brigitte; Terstappen, Leon; Repollet, Madeline; Pierga, Jean-Yves; Miller, Craig; Sotiriou, Christos; Michiels, Stefan; Pantel, Klaus.
In: BREAST CANCER RES, Vol. 16, No. 2, 01.01.2014, p. R43.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - International study on inter-reader variability for circulating tumor cells in breast cancer
AU - Ignatiadis, Michail
AU - Riethdorf, Sabine
AU - Bidard, François-Clement
AU - Vaucher, Isabelle
AU - Khazour, Mustapha
AU - Rothé, Françoise
AU - Metallo, Jessica
AU - Rouas, Ghizlane
AU - Payne, Rachel E
AU - Coombes, Raoul
AU - Teufel, Ingrid
AU - Andergassen, Ulrich
AU - Apostolaki, Stella
AU - Politaki, Eleni
AU - Mavroudis, Dimitris
AU - Bessi, Silvia
AU - Pestrin, Marta
AU - Di Leo, Angelo
AU - Campion, Michael
AU - Reinholz, Monica
AU - Perez, Edith
AU - Piccart, Martine
AU - Borgen, Elin
AU - Naume, Bjorn
AU - Jimenez, Jose
AU - Aura, Claudia
AU - Zorzino, Laura
AU - Cassatella, Maria
AU - Sandri, Maria
AU - Mostert, Bianca
AU - Sleijfer, Stefan
AU - Kraan, Jaco
AU - Janni, Wolfgang
AU - Fehm, Tanja
AU - Rack, Brigitte
AU - Terstappen, Leon
AU - Repollet, Madeline
AU - Pierga, Jean-Yves
AU - Miller, Craig
AU - Sotiriou, Christos
AU - Michiels, Stefan
AU - Pantel, Klaus
PY - 2014/1/1
Y1 - 2014/1/1
N2 - INTRODUCTION: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.METHODS: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.RESULTS: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).CONCLUSIONS: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.
AB - INTRODUCTION: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.METHODS: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.RESULTS: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).CONCLUSIONS: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.
KW - Breast Neoplasms
KW - Cell Count
KW - Female
KW - Humans
KW - International Cooperation
KW - Laboratories
KW - Medical Oncology
KW - Neoplasm Metastasis
KW - Neoplastic Cells, Circulating
KW - Receptor, ErbB-2
KW - Reference Standards
KW - Reproducibility of Results
U2 - 10.1186/bcr3647
DO - 10.1186/bcr3647
M3 - SCORING: Journal article
C2 - 24758318
VL - 16
SP - R43
JO - BREAST CANCER RES
JF - BREAST CANCER RES
SN - 1465-5411
IS - 2
ER -