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International study on inter-reader variability for circulating tumor cells in breast cancer

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International study on inter-reader variability for circulating tumor cells in breast cancer. / Ignatiadis, Michail; Riethdorf, Sabine; Bidard, François-Clement; Vaucher, Isabelle; Khazour, Mustapha; Rothé, Françoise; Metallo, Jessica; Rouas, Ghizlane; Payne, Rachel E; Coombes, Raoul; Teufel, Ingrid; Andergassen, Ulrich; Apostolaki, Stella; Politaki, Eleni; Mavroudis, Dimitris; Bessi, Silvia; Pestrin, Marta; Di Leo, Angelo; Campion, Michael; Reinholz, Monica; Perez, Edith; Piccart, Martine; Borgen, Elin; Naume, Bjorn; Jimenez, Jose; Aura, Claudia; Zorzino, Laura; Cassatella, Maria; Sandri, Maria; Mostert, Bianca; Sleijfer, Stefan; Kraan, Jaco; Janni, Wolfgang; Fehm, Tanja; Rack, Brigitte; Terstappen, Leon; Repollet, Madeline; Pierga, Jean-Yves; Miller, Craig; Sotiriou, Christos; Michiels, Stefan; Pantel, Klaus.

in: BREAST CANCER RES, Jahrgang 16, Nr. 2, 01.01.2014, S. R43.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ignatiadis, M, Riethdorf, S, Bidard, F-C, Vaucher, I, Khazour, M, Rothé, F, Metallo, J, Rouas, G, Payne, RE, Coombes, R, Teufel, I, Andergassen, U, Apostolaki, S, Politaki, E, Mavroudis, D, Bessi, S, Pestrin, M, Di Leo, A, Campion, M, Reinholz, M, Perez, E, Piccart, M, Borgen, E, Naume, B, Jimenez, J, Aura, C, Zorzino, L, Cassatella, M, Sandri, M, Mostert, B, Sleijfer, S, Kraan, J, Janni, W, Fehm, T, Rack, B, Terstappen, L, Repollet, M, Pierga, J-Y, Miller, C, Sotiriou, C, Michiels, S & Pantel, K 2014, 'International study on inter-reader variability for circulating tumor cells in breast cancer', BREAST CANCER RES, Jg. 16, Nr. 2, S. R43. https://doi.org/10.1186/bcr3647

APA

Ignatiadis, M., Riethdorf, S., Bidard, F-C., Vaucher, I., Khazour, M., Rothé, F., Metallo, J., Rouas, G., Payne, R. E., Coombes, R., Teufel, I., Andergassen, U., Apostolaki, S., Politaki, E., Mavroudis, D., Bessi, S., Pestrin, M., Di Leo, A., Campion, M., ... Pantel, K. (2014). International study on inter-reader variability for circulating tumor cells in breast cancer. BREAST CANCER RES, 16(2), R43. https://doi.org/10.1186/bcr3647

Vancouver

Ignatiadis M, Riethdorf S, Bidard F-C, Vaucher I, Khazour M, Rothé F et al. International study on inter-reader variability for circulating tumor cells in breast cancer. BREAST CANCER RES. 2014 Jan 1;16(2):R43. https://doi.org/10.1186/bcr3647

Bibtex

@article{5c530fd368f34d10b789eaea83f2e0ac,
title = "International study on inter-reader variability for circulating tumor cells in breast cancer",
abstract = "INTRODUCTION: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch{\textregistered} system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.METHODS: CellSearch{\textregistered} images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.RESULTS: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).CONCLUSIONS: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.",
keywords = "Breast Neoplasms, Cell Count, Female, Humans, International Cooperation, Laboratories, Medical Oncology, Neoplasm Metastasis, Neoplastic Cells, Circulating, Receptor, ErbB-2, Reference Standards, Reproducibility of Results",
author = "Michail Ignatiadis and Sabine Riethdorf and Fran{\c c}ois-Clement Bidard and Isabelle Vaucher and Mustapha Khazour and Fran{\c c}oise Roth{\'e} and Jessica Metallo and Ghizlane Rouas and Payne, {Rachel E} and Raoul Coombes and Ingrid Teufel and Ulrich Andergassen and Stella Apostolaki and Eleni Politaki and Dimitris Mavroudis and Silvia Bessi and Marta Pestrin and {Di Leo}, Angelo and Michael Campion and Monica Reinholz and Edith Perez and Martine Piccart and Elin Borgen and Bjorn Naume and Jose Jimenez and Claudia Aura and Laura Zorzino and Maria Cassatella and Maria Sandri and Bianca Mostert and Stefan Sleijfer and Jaco Kraan and Wolfgang Janni and Tanja Fehm and Brigitte Rack and Leon Terstappen and Madeline Repollet and Jean-Yves Pierga and Craig Miller and Christos Sotiriou and Stefan Michiels and Klaus Pantel",
year = "2014",
month = jan,
day = "1",
doi = "10.1186/bcr3647",
language = "English",
volume = "16",
pages = "R43",
journal = "BREAST CANCER RES",
issn = "1465-5411",
publisher = "BioMed Central Ltd.",
number = "2",

}

RIS

TY - JOUR

T1 - International study on inter-reader variability for circulating tumor cells in breast cancer

AU - Ignatiadis, Michail

AU - Riethdorf, Sabine

AU - Bidard, François-Clement

AU - Vaucher, Isabelle

AU - Khazour, Mustapha

AU - Rothé, Françoise

AU - Metallo, Jessica

AU - Rouas, Ghizlane

AU - Payne, Rachel E

AU - Coombes, Raoul

AU - Teufel, Ingrid

AU - Andergassen, Ulrich

AU - Apostolaki, Stella

AU - Politaki, Eleni

AU - Mavroudis, Dimitris

AU - Bessi, Silvia

AU - Pestrin, Marta

AU - Di Leo, Angelo

AU - Campion, Michael

AU - Reinholz, Monica

AU - Perez, Edith

AU - Piccart, Martine

AU - Borgen, Elin

AU - Naume, Bjorn

AU - Jimenez, Jose

AU - Aura, Claudia

AU - Zorzino, Laura

AU - Cassatella, Maria

AU - Sandri, Maria

AU - Mostert, Bianca

AU - Sleijfer, Stefan

AU - Kraan, Jaco

AU - Janni, Wolfgang

AU - Fehm, Tanja

AU - Rack, Brigitte

AU - Terstappen, Leon

AU - Repollet, Madeline

AU - Pierga, Jean-Yves

AU - Miller, Craig

AU - Sotiriou, Christos

AU - Michiels, Stefan

AU - Pantel, Klaus

PY - 2014/1/1

Y1 - 2014/1/1

N2 - INTRODUCTION: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.METHODS: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.RESULTS: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).CONCLUSIONS: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.

AB - INTRODUCTION: Circulating tumor cells (CTCs) have been studied in breast cancer with the CellSearch® system. Given the low CTC counts in non-metastatic breast cancer, it is important to evaluate the inter-reader agreement.METHODS: CellSearch® images (N = 272) of either CTCs or white blood cells or artifacts from 109 non-metastatic (M0) and 22 metastatic (M1) breast cancer patients from reported studies were sent to 22 readers from 15 academic laboratories and 8 readers from two Veridex laboratories. Each image was scored as No CTC vs CTC HER2- vs CTC HER2+. The 8 Veridex readers were summarized to a Veridex Consensus (VC) to compare each academic reader using % agreement and kappa (κ) statistics. Agreement was compared according to disease stage and CTC counts using the Wilcoxon signed rank test.RESULTS: For CTC definition (No CTC vs CTC), the median agreement between academic readers and VC was 92% (range 69 to 97%) with a median κ of 0.83 (range 0.37 to 0.93). Lower agreement was observed in images from M0 (median 91%, range 70 to 96%) compared to M1 (median 98%, range 64 to 100%) patients (P < 0.001) and from M0 and <3CTCs (median 87%, range 66 to 95%) compared to M0 and ≥3CTCs samples (median 95%, range 77 to 99%), (P < 0.001). For CTC HER2 expression (HER2- vs HER2+), the median agreement was 87% (range 51 to 95%) with a median κ of 0.74 (range 0.25 to 0.90).CONCLUSIONS: The inter-reader agreement for CTC definition was high. Reduced agreement was observed in M0 patients with low CTC counts. Continuous training and independent image review are required.

KW - Breast Neoplasms

KW - Cell Count

KW - Female

KW - Humans

KW - International Cooperation

KW - Laboratories

KW - Medical Oncology

KW - Neoplasm Metastasis

KW - Neoplastic Cells, Circulating

KW - Receptor, ErbB-2

KW - Reference Standards

KW - Reproducibility of Results

U2 - 10.1186/bcr3647

DO - 10.1186/bcr3647

M3 - SCORING: Journal article

C2 - 24758318

VL - 16

SP - R43

JO - BREAST CANCER RES

JF - BREAST CANCER RES

SN - 1465-5411

IS - 2

ER -