Interleukin 1 and tumor necrosis factor stimulate cGMP formation in rat renal mesangial cells
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Interleukin 1 and tumor necrosis factor stimulate cGMP formation in rat renal mesangial cells. / Pfeilschifter, J; Schwarzenbach, H.
In: FEBS LETT, Vol. 273, No. 1-2, 29.10.1990, p. 185-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Interleukin 1 and tumor necrosis factor stimulate cGMP formation in rat renal mesangial cells
AU - Pfeilschifter, J
AU - Schwarzenbach, H
PY - 1990/10/29
Y1 - 1990/10/29
N2 - Treatment of mesangial cells with recombinant human interleukin 1 beta (IL-1 beta) or recombinant human tumor necrosis factor alpha (TNF alpha) dose-dependently increased cGMP formation. Both IL-1 beta and TNF alpha-stimulated formation of cGMP occurred after a initial lag period of 4 to 8 hours. Treatment of cells with actinomycin D, cycloheximide or dexamethason completely abolished cytokine-induced cGMP formation. Furthermore, the guanylate cyclase inhibitor Methylene blue completely blocked IL-1 beta- and TNF alpha-stimulated cGMP generation. NG-mono-methyl-L-arginine attenuated IL-1 beta- and TNF alpha-induced cGMP production, an effect that was reversed by L-arginine.
AB - Treatment of mesangial cells with recombinant human interleukin 1 beta (IL-1 beta) or recombinant human tumor necrosis factor alpha (TNF alpha) dose-dependently increased cGMP formation. Both IL-1 beta and TNF alpha-stimulated formation of cGMP occurred after a initial lag period of 4 to 8 hours. Treatment of cells with actinomycin D, cycloheximide or dexamethason completely abolished cytokine-induced cGMP formation. Furthermore, the guanylate cyclase inhibitor Methylene blue completely blocked IL-1 beta- and TNF alpha-stimulated cGMP generation. NG-mono-methyl-L-arginine attenuated IL-1 beta- and TNF alpha-induced cGMP production, an effect that was reversed by L-arginine.
KW - Animals
KW - Cells, Cultured
KW - Cyclic GMP
KW - Glomerular Mesangium
KW - Interleukin-1
KW - Kinetics
KW - Rats
KW - Recombinant Proteins
KW - Tumor Necrosis Factor-alpha
M3 - SCORING: Journal article
C2 - 2172027
VL - 273
SP - 185
EP - 187
JO - FEBS LETT
JF - FEBS LETT
SN - 0014-5793
IS - 1-2
ER -