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Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency

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Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency. / Ainsbury, Elizabeth A; Al-Hafidh, Jenna; Bajinskis, Ainars; Barnard, Stephen; Barquinero, Joan Francesc; Beinke, Christina; de Gelder, Virginie; Gregoire, Eric; Jaworska, Alicja; Lindholm, Carita; Lloyd, David; Moquet, Jayne; Nylund, Reetta; Oestreicher, Ursula; Roch-Lefévre, Sandrine; Rothkamm, Kai; Romm, Horst; Scherthan, Harry; Sommer, Sylwester; Thierens, Hubert; Vandevoorde, Charlot; Vral, Anne; Wojcik, Andrzej.

In: INT J RADIAT BIOL, Vol. 90, No. 2, 02.2014, p. 193-202.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ainsbury, EA, Al-Hafidh, J, Bajinskis, A, Barnard, S, Barquinero, JF, Beinke, C, de Gelder, V, Gregoire, E, Jaworska, A, Lindholm, C, Lloyd, D, Moquet, J, Nylund, R, Oestreicher, U, Roch-Lefévre, S, Rothkamm, K, Romm, H, Scherthan, H, Sommer, S, Thierens, H, Vandevoorde, C, Vral, A & Wojcik, A 2014, 'Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency', INT J RADIAT BIOL, vol. 90, no. 2, pp. 193-202. https://doi.org/10.3109/09553002.2014.868616

APA

Ainsbury, E. A., Al-Hafidh, J., Bajinskis, A., Barnard, S., Barquinero, J. F., Beinke, C., de Gelder, V., Gregoire, E., Jaworska, A., Lindholm, C., Lloyd, D., Moquet, J., Nylund, R., Oestreicher, U., Roch-Lefévre, S., Rothkamm, K., Romm, H., Scherthan, H., Sommer, S., ... Wojcik, A. (2014). Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency. INT J RADIAT BIOL, 90(2), 193-202. https://doi.org/10.3109/09553002.2014.868616

Vancouver

Ainsbury EA, Al-Hafidh J, Bajinskis A, Barnard S, Barquinero JF, Beinke C et al. Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency. INT J RADIAT BIOL. 2014 Feb;90(2):193-202. https://doi.org/10.3109/09553002.2014.868616

Bibtex

@article{a9aebd7e68ac48078747d30679821b7f,
title = "Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency",
abstract = "PURPOSE: The European Union's Seventh Framework Programme-funded project 'Multi-disciplinary biodosimetric tools to manage high scale radiological casualties' (MULTIBIODOSE) has developed a multiparametric approach to radiation biodosimetry, with a particular emphasis on triage of large numbers of potentially exposed individuals following accidental exposures. In November 2012, an emergency exercise took place which tested the capabilities of the MULTIBIODOSE project partners. The exercise described here had a dual purpose: Intercomparison of (i) three biodosimetric assays, and (ii) the capabilities of the seven laboratories, with regards to provision of triage status for suspected radiation exposed individuals.MATERIALS AND METHODS: Three biological dosimetry tools - the dicentric, micronucleus and gamma-H2AX (the phosphorylated form of member X of histone H2A, in response to DNA double-strand breaks) foci assays - were tested, in addition to provision of the triage status results (low exposure: < 1 Gy; medium exposure: 1-2 Gy; high exposure: > 2 Gy) by the MULTIBIODOSE software. The exercise was run in two modes: An initial triage categorisation of samples (based on the first dose estimates for each assay received from each laboratory) followed by collation of the full set of estimated doses (all the results from all modes of each assay carried out by the participating laboratories) calculated using as many modes of operation as possible of the different assays developed during the project. Simulated acute whole body and partial body exposures were included.RESULTS: The results of the initial triage categorisation and the full comparison of assays and methods within and between laboratories are presented here.CONCLUSIONS: The data demonstrate that the MULTIBIODOSE approach of applying multiparametric tools to radiation emergencies is valid and effective.",
keywords = "Histones/analysis, Humans, Laboratories, Micronucleus Tests, Radiometry, Time Factors, Triage",
author = "Ainsbury, {Elizabeth A} and Jenna Al-Hafidh and Ainars Bajinskis and Stephen Barnard and Barquinero, {Joan Francesc} and Christina Beinke and {de Gelder}, Virginie and Eric Gregoire and Alicja Jaworska and Carita Lindholm and David Lloyd and Jayne Moquet and Reetta Nylund and Ursula Oestreicher and Sandrine Roch-Lef{\'e}vre and Kai Rothkamm and Horst Romm and Harry Scherthan and Sylwester Sommer and Hubert Thierens and Charlot Vandevoorde and Anne Vral and Andrzej Wojcik",
year = "2014",
month = feb,
doi = "10.3109/09553002.2014.868616",
language = "English",
volume = "90",
pages = "193--202",
journal = "INT J RADIAT BIOL",
issn = "0955-3002",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Inter- and intra-laboratory comparison of a multibiodosimetric approach to triage in a simulated, large scale radiation emergency

AU - Ainsbury, Elizabeth A

AU - Al-Hafidh, Jenna

AU - Bajinskis, Ainars

AU - Barnard, Stephen

AU - Barquinero, Joan Francesc

AU - Beinke, Christina

AU - de Gelder, Virginie

AU - Gregoire, Eric

AU - Jaworska, Alicja

AU - Lindholm, Carita

AU - Lloyd, David

AU - Moquet, Jayne

AU - Nylund, Reetta

AU - Oestreicher, Ursula

AU - Roch-Lefévre, Sandrine

AU - Rothkamm, Kai

AU - Romm, Horst

AU - Scherthan, Harry

AU - Sommer, Sylwester

AU - Thierens, Hubert

AU - Vandevoorde, Charlot

AU - Vral, Anne

AU - Wojcik, Andrzej

PY - 2014/2

Y1 - 2014/2

N2 - PURPOSE: The European Union's Seventh Framework Programme-funded project 'Multi-disciplinary biodosimetric tools to manage high scale radiological casualties' (MULTIBIODOSE) has developed a multiparametric approach to radiation biodosimetry, with a particular emphasis on triage of large numbers of potentially exposed individuals following accidental exposures. In November 2012, an emergency exercise took place which tested the capabilities of the MULTIBIODOSE project partners. The exercise described here had a dual purpose: Intercomparison of (i) three biodosimetric assays, and (ii) the capabilities of the seven laboratories, with regards to provision of triage status for suspected radiation exposed individuals.MATERIALS AND METHODS: Three biological dosimetry tools - the dicentric, micronucleus and gamma-H2AX (the phosphorylated form of member X of histone H2A, in response to DNA double-strand breaks) foci assays - were tested, in addition to provision of the triage status results (low exposure: < 1 Gy; medium exposure: 1-2 Gy; high exposure: > 2 Gy) by the MULTIBIODOSE software. The exercise was run in two modes: An initial triage categorisation of samples (based on the first dose estimates for each assay received from each laboratory) followed by collation of the full set of estimated doses (all the results from all modes of each assay carried out by the participating laboratories) calculated using as many modes of operation as possible of the different assays developed during the project. Simulated acute whole body and partial body exposures were included.RESULTS: The results of the initial triage categorisation and the full comparison of assays and methods within and between laboratories are presented here.CONCLUSIONS: The data demonstrate that the MULTIBIODOSE approach of applying multiparametric tools to radiation emergencies is valid and effective.

AB - PURPOSE: The European Union's Seventh Framework Programme-funded project 'Multi-disciplinary biodosimetric tools to manage high scale radiological casualties' (MULTIBIODOSE) has developed a multiparametric approach to radiation biodosimetry, with a particular emphasis on triage of large numbers of potentially exposed individuals following accidental exposures. In November 2012, an emergency exercise took place which tested the capabilities of the MULTIBIODOSE project partners. The exercise described here had a dual purpose: Intercomparison of (i) three biodosimetric assays, and (ii) the capabilities of the seven laboratories, with regards to provision of triage status for suspected radiation exposed individuals.MATERIALS AND METHODS: Three biological dosimetry tools - the dicentric, micronucleus and gamma-H2AX (the phosphorylated form of member X of histone H2A, in response to DNA double-strand breaks) foci assays - were tested, in addition to provision of the triage status results (low exposure: < 1 Gy; medium exposure: 1-2 Gy; high exposure: > 2 Gy) by the MULTIBIODOSE software. The exercise was run in two modes: An initial triage categorisation of samples (based on the first dose estimates for each assay received from each laboratory) followed by collation of the full set of estimated doses (all the results from all modes of each assay carried out by the participating laboratories) calculated using as many modes of operation as possible of the different assays developed during the project. Simulated acute whole body and partial body exposures were included.RESULTS: The results of the initial triage categorisation and the full comparison of assays and methods within and between laboratories are presented here.CONCLUSIONS: The data demonstrate that the MULTIBIODOSE approach of applying multiparametric tools to radiation emergencies is valid and effective.

KW - Histones/analysis

KW - Humans

KW - Laboratories

KW - Micronucleus Tests

KW - Radiometry

KW - Time Factors

KW - Triage

U2 - 10.3109/09553002.2014.868616

DO - 10.3109/09553002.2014.868616

M3 - SCORING: Journal article

C2 - 24289146

VL - 90

SP - 193

EP - 202

JO - INT J RADIAT BIOL

JF - INT J RADIAT BIOL

SN - 0955-3002

IS - 2

ER -