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Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates

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Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates. / Maenz, Claudia; Loscher, Christine; Iwanski, Alicja; Bruns, Michael.

In: J GEN VIROL, Vol. 89, No. Pt 12, 12.2008, p. 3016-26.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Maenz, C, Loscher, C, Iwanski, A & Bruns, M 2008, 'Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates', J GEN VIROL, vol. 89, no. Pt 12, pp. 3016-26. https://doi.org/10.1099/vir.0.2008/003541-0

APA

Maenz, C., Loscher, C., Iwanski, A., & Bruns, M. (2008). Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates. J GEN VIROL, 89(Pt 12), 3016-26. https://doi.org/10.1099/vir.0.2008/003541-0

Vancouver

Maenz C, Loscher C, Iwanski A, Bruns M. Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates. J GEN VIROL. 2008 Dec;89(Pt 12):3016-26. https://doi.org/10.1099/vir.0.2008/003541-0

Bibtex

@article{39dcf1bc27ba4d80b8eaa5d8081522ab,
title = "Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates",
abstract = "The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.",
keywords = "Animals, Carbohydrates, Cells, Cultured, Ducks, Hepadnaviridae Infections, Hepatitis B Virus, Duck, Hepatitis B e Antigens, Hepatitis, Viral, Animal, Hepatocytes, Liver, Methylmannosides, Virus Replication",
author = "Claudia Maenz and Christine Loscher and Alicja Iwanski and Michael Bruns",
year = "2008",
month = dec,
doi = "10.1099/vir.0.2008/003541-0",
language = "English",
volume = "89",
pages = "3016--26",
journal = "J GEN VIROL",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "Pt 12",

}

RIS

TY - JOUR

T1 - Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates

AU - Maenz, Claudia

AU - Loscher, Christine

AU - Iwanski, Alicja

AU - Bruns, Michael

PY - 2008/12

Y1 - 2008/12

N2 - The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.

AB - The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.

KW - Animals

KW - Carbohydrates

KW - Cells, Cultured

KW - Ducks

KW - Hepadnaviridae Infections

KW - Hepatitis B Virus, Duck

KW - Hepatitis B e Antigens

KW - Hepatitis, Viral, Animal

KW - Hepatocytes

KW - Liver

KW - Methylmannosides

KW - Virus Replication

U2 - 10.1099/vir.0.2008/003541-0

DO - 10.1099/vir.0.2008/003541-0

M3 - SCORING: Journal article

C2 - 19008388

VL - 89

SP - 3016

EP - 3026

JO - J GEN VIROL

JF - J GEN VIROL

SN - 0022-1317

IS - Pt 12

ER -