Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates
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Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates. / Maenz, Claudia; Loscher, Christine; Iwanski, Alicja; Bruns, Michael.
in: J GEN VIROL, Jahrgang 89, Nr. Pt 12, 12.2008, S. 3016-26.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Inhibition of duck hepatitis B virus infection of liver cells by combined treatment with viral e antigen and carbohydrates
AU - Maenz, Claudia
AU - Loscher, Christine
AU - Iwanski, Alicja
AU - Bruns, Michael
PY - 2008/12
Y1 - 2008/12
N2 - The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.
AB - The e antigen (eAg) of duck hepatitis B virus (DHBV) is a glycosylated secretory protein with a currently unknown function. We concentrated this antigen from the supernatants of persistently infected primary duck liver cell cultures by ammonium sulphate precipitation, adsorption chromatography over concanavalin A Sepharose, preparative isoelectric focusing and molecular sieve chromatography. The combined treatment of duck liver cells with DHBV eAg (DHBe) concentrate and alpha-methyl-d-mannopyranoside strongly inhibited DHBV replication at de novo infection. When DHBe was added to non-infected primary duck liver cells, it was found to be associated with liver sinusoidal endothelial cells. This binding could be inhibited by the addition of alpha-methyl-d-mannopyranoside and other sugar molecules. The inhibitory effect of DHBe on infection could play a role in maintaining viral persistence.
KW - Animals
KW - Carbohydrates
KW - Cells, Cultured
KW - Ducks
KW - Hepadnaviridae Infections
KW - Hepatitis B Virus, Duck
KW - Hepatitis B e Antigens
KW - Hepatitis, Viral, Animal
KW - Hepatocytes
KW - Liver
KW - Methylmannosides
KW - Virus Replication
U2 - 10.1099/vir.0.2008/003541-0
DO - 10.1099/vir.0.2008/003541-0
M3 - SCORING: Journal article
C2 - 19008388
VL - 89
SP - 3016
EP - 3026
JO - J GEN VIROL
JF - J GEN VIROL
SN - 0022-1317
IS - Pt 12
ER -