Inhibition of casein kinase I delta alters mitotic spindle formation and induces apoptosis in trophoblast cells.
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Inhibition of casein kinase I delta alters mitotic spindle formation and induces apoptosis in trophoblast cells. / Stöter, Martin; Bamberger, Ana-Maria; Aslan, Bilnaz; Kurth, Marion; Speidel, Daniel; Löning, Thomas; Frank, Hans-Georg; Kaufmann, Peter; Löhler, Jürgen; Henne-Bruns, Doris; Deppert, Wolfgang; Knippschild, Uwe.
In: ONCOGENE, Vol. 24, No. 54, 54, 2005, p. 7964-7975.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Inhibition of casein kinase I delta alters mitotic spindle formation and induces apoptosis in trophoblast cells.
AU - Stöter, Martin
AU - Bamberger, Ana-Maria
AU - Aslan, Bilnaz
AU - Kurth, Marion
AU - Speidel, Daniel
AU - Löning, Thomas
AU - Frank, Hans-Georg
AU - Kaufmann, Peter
AU - Löhler, Jürgen
AU - Henne-Bruns, Doris
AU - Deppert, Wolfgang
AU - Knippschild, Uwe
PY - 2005
Y1 - 2005
N2 - The serine/threonine-specific casein kinase I delta (CKIdelta) is ubiquitously expressed in all tissues, is p53 dependently induced in stress situations and plays an important role in various cellular processes. Our immunohistochemical analysis of the human placenta revealed strongest expression of CKIdelta in extravillous trophoblast cells and in choriocarcinomas. Investigation of the functional role of CKIdelta in an extravillous trophoblast hybrid cell line revealed that CKIdelta was constitutively localized at the centrosomes and the mitotic spindle. Inhibition of CKIdelta with the CKI-specific inhibitor IC261 led to structural alterations of the centrosomes, the formation of multipolar spindles, the inhibition of mitosis and, in contrast to other cell lines, the induction of apoptosis. Our findings indicate that CKIdelta plays an important role in the mitotic progression and in the survival of cells of trophoblast origin. Therefore, IC261 could provide a new tool in treating choriocarcinomas.
AB - The serine/threonine-specific casein kinase I delta (CKIdelta) is ubiquitously expressed in all tissues, is p53 dependently induced in stress situations and plays an important role in various cellular processes. Our immunohistochemical analysis of the human placenta revealed strongest expression of CKIdelta in extravillous trophoblast cells and in choriocarcinomas. Investigation of the functional role of CKIdelta in an extravillous trophoblast hybrid cell line revealed that CKIdelta was constitutively localized at the centrosomes and the mitotic spindle. Inhibition of CKIdelta with the CKI-specific inhibitor IC261 led to structural alterations of the centrosomes, the formation of multipolar spindles, the inhibition of mitosis and, in contrast to other cell lines, the induction of apoptosis. Our findings indicate that CKIdelta plays an important role in the mitotic progression and in the survival of cells of trophoblast origin. Therefore, IC261 could provide a new tool in treating choriocarcinomas.
M3 - SCORING: Zeitschriftenaufsatz
VL - 24
SP - 7964
EP - 7975
JO - ONCOGENE
JF - ONCOGENE
SN - 0950-9232
IS - 54
M1 - 54
ER -