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Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells

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Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. / Drengk, Anja; Zapf, Antonia; Stürmer, Ewa Klara; Stürmer, Klaus Michael; Frosch, Karl-Heinz.

In: CELLS TISSUES ORGANS, Vol. 189, No. 5, 2009, p. 317-326.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Drengk, A, Zapf, A, Stürmer, EK, Stürmer, KM & Frosch, K-H 2009, 'Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells', CELLS TISSUES ORGANS, vol. 189, no. 5, pp. 317-326. https://doi.org/10.1159/000151290

APA

Drengk, A., Zapf, A., Stürmer, E. K., Stürmer, K. M., & Frosch, K-H. (2009). Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. CELLS TISSUES ORGANS, 189(5), 317-326. https://doi.org/10.1159/000151290

Vancouver

Drengk A, Zapf A, Stürmer EK, Stürmer KM, Frosch K-H. Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. CELLS TISSUES ORGANS. 2009;189(5):317-326. https://doi.org/10.1159/000151290

Bibtex

@article{fa0a0297e3e44ad4ba2a20790a3f75f2,
title = "Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells",
abstract = "BACKGROUND/AIMS: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC).METHODS: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining.RESULTS: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity.CONCLUSION: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required.",
keywords = "Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Chondrocytes, Female, Immunohistochemistry, Mesenchymal Stromal Cells, Platelet-Rich Plasma, Reverse Transcriptase Polymerase Chain Reaction, Sheep, Journal Article",
author = "Anja Drengk and Antonia Zapf and St{\"u}rmer, {Ewa Klara} and St{\"u}rmer, {Klaus Michael} and Karl-Heinz Frosch",
note = "2008 S. Karger AG, Basel.",
year = "2009",
doi = "10.1159/000151290",
language = "English",
volume = "189",
pages = "317--326",
journal = "CELLS TISSUES ORGANS",
issn = "1422-6405",
publisher = "S. Karger AG",
number = "5",

}

RIS

TY - JOUR

T1 - Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells

AU - Drengk, Anja

AU - Zapf, Antonia

AU - Stürmer, Ewa Klara

AU - Stürmer, Klaus Michael

AU - Frosch, Karl-Heinz

N1 - 2008 S. Karger AG, Basel.

PY - 2009

Y1 - 2009

N2 - BACKGROUND/AIMS: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC).METHODS: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining.RESULTS: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity.CONCLUSION: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required.

AB - BACKGROUND/AIMS: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC).METHODS: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining.RESULTS: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity.CONCLUSION: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required.

KW - Animals

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cells, Cultured

KW - Chondrocytes

KW - Female

KW - Immunohistochemistry

KW - Mesenchymal Stromal Cells

KW - Platelet-Rich Plasma

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sheep

KW - Journal Article

U2 - 10.1159/000151290

DO - 10.1159/000151290

M3 - SCORING: Journal article

C2 - 18689989

VL - 189

SP - 317

EP - 326

JO - CELLS TISSUES ORGANS

JF - CELLS TISSUES ORGANS

SN - 1422-6405

IS - 5

ER -