Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells
Standard
Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells. / Drengk, Anja; Zapf, Antonia; Stürmer, Ewa Klara; Stürmer, Klaus Michael; Frosch, Karl-Heinz.
in: CELLS TISSUES ORGANS, Jahrgang 189, Nr. 5, 2009, S. 317-326.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Influence of platelet-rich plasma on chondrogenic differentiation and proliferation of chondrocytes and mesenchymal stem cells
AU - Drengk, Anja
AU - Zapf, Antonia
AU - Stürmer, Ewa Klara
AU - Stürmer, Klaus Michael
AU - Frosch, Karl-Heinz
N1 - 2008 S. Karger AG, Basel.
PY - 2009
Y1 - 2009
N2 - BACKGROUND/AIMS: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC).METHODS: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining.RESULTS: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity.CONCLUSION: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required.
AB - BACKGROUND/AIMS: Autologous chondrocyte (CC) transplantation has the disadvantages of requiring two surgical interventions and in vitro expansion of cells, implying the risk of cellular dedifferentiation. Our clinical aim is to develop a one-step procedure for autologous CC transplantation, i.e. harvesting, isolation and reimplantation of CC performed in one single surgical procedure. Platelet-rich plasma (PRP) is a source of autologous growth factors reported to have mitogenic effects. The objective of this study was to test the influence of PRP as an autologous scaffold on freshly isolated CC and mesenchymal stem cells (MSC).METHODS: CC and MSC were subjected to two- or three-dimensional (3D) growth systems, either with or without PRP. Chondrogenic differentiation was determined via quantification of collagen type II mRNA and immunohistochemical staining.RESULTS: We observed a proliferative effect for MSCs exposed to PRP in monolayer culture and an increase in the expression of chondrogenic markers when cells are exposed to a 3D environment. CCs exposed to PRP show a decrease in the chondrogenic phenotype with increasing proliferative activity.CONCLUSION: PRP has a proliferative effect on CCs and MSCs. In a one-step procedure for autologous CC transplantation, this might be an advantage over other scaffold materials, but confirmation in in vivo studies is required.
KW - Animals
KW - Cell Differentiation
KW - Cell Proliferation
KW - Cells, Cultured
KW - Chondrocytes
KW - Female
KW - Immunohistochemistry
KW - Mesenchymal Stromal Cells
KW - Platelet-Rich Plasma
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Sheep
KW - Journal Article
U2 - 10.1159/000151290
DO - 10.1159/000151290
M3 - SCORING: Journal article
C2 - 18689989
VL - 189
SP - 317
EP - 326
JO - CELLS TISSUES ORGANS
JF - CELLS TISSUES ORGANS
SN - 1422-6405
IS - 5
ER -