Inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models
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Inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models. / Stubbendorff, Mandy; Hua, Xiaoqin; Deuse, Tobias; Ali, Ziad; Reichenspurner, Hermann; Maegdefessel, Lars; Robbins, Robert C; Schrepfer, Sonja.
In: JOVE-J VIS EXP, No. 87, 14.05.2014, p. e51459.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Inducing myointimal hyperplasia versus atherosclerosis in mice: an introduction of two valid models
AU - Stubbendorff, Mandy
AU - Hua, Xiaoqin
AU - Deuse, Tobias
AU - Ali, Ziad
AU - Reichenspurner, Hermann
AU - Maegdefessel, Lars
AU - Robbins, Robert C
AU - Schrepfer, Sonja
PY - 2014/5/14
Y1 - 2014/5/14
N2 - Various in vivo laboratory rodent models for the induction of artery stenosis have been established to mimic diseases that include arterial plaque formation and stenosis, as observed for example in ischemic heart disease. Two highly reproducible mouse models - both resulting in artery stenosis but each underlying a different pathway of development - are introduced here. The models represent the two most common causes of artery stenosis; namely one mouse model for each myointimal hyperplasia, and atherosclerosis are shown. To induce myointimal hyperplasia, a balloon catheter injury of the abdominal aorta is performed. For the development of atherosclerotic plaque, the ApoE -/- mouse model in combination with western fatty diet is used. Different model-adapted options for the measurement and evaluation of the results are named and described in this manuscript. The introduction and comparison of these two models provides information for scientists to choose the appropriate artery stenosis model in accordance to the scientific question asked.
AB - Various in vivo laboratory rodent models for the induction of artery stenosis have been established to mimic diseases that include arterial plaque formation and stenosis, as observed for example in ischemic heart disease. Two highly reproducible mouse models - both resulting in artery stenosis but each underlying a different pathway of development - are introduced here. The models represent the two most common causes of artery stenosis; namely one mouse model for each myointimal hyperplasia, and atherosclerosis are shown. To induce myointimal hyperplasia, a balloon catheter injury of the abdominal aorta is performed. For the development of atherosclerotic plaque, the ApoE -/- mouse model in combination with western fatty diet is used. Different model-adapted options for the measurement and evaluation of the results are named and described in this manuscript. The introduction and comparison of these two models provides information for scientists to choose the appropriate artery stenosis model in accordance to the scientific question asked.
KW - Animals
KW - Apolipoproteins E/deficiency
KW - Atherosclerosis/pathology
KW - Disease Models, Animal
KW - Female
KW - Hyperplasia/pathology
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Plaque, Atherosclerotic/pathology
KW - Reproducibility of Results
KW - Tunica Intima/pathology
U2 - 10.3791/51459
DO - 10.3791/51459
M3 - SCORING: Journal article
C2 - 24893977
SP - e51459
JO - JOVE-J VIS EXP
JF - JOVE-J VIS EXP
SN - 1940-087X
IS - 87
ER -