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In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature

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In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature. / Bartsch, F; Kang, M L; Mees, S T; Haier, J; Gassmann, P.

In: Methods Mol Biol, Vol. 1066, 2013, p. 89-101.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bartsch, F, Kang, ML, Mees, ST, Haier, J & Gassmann, P 2013, 'In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature', Methods Mol Biol, vol. 1066, pp. 89-101. https://doi.org/10.1007/978-1-62703-604-7_8

APA

Bartsch, F., Kang, M. L., Mees, S. T., Haier, J., & Gassmann, P. (2013). In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature. Methods Mol Biol, 1066, 89-101. https://doi.org/10.1007/978-1-62703-604-7_8

Vancouver

Bartsch F, Kang ML, Mees ST, Haier J, Gassmann P. In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature. Methods Mol Biol. 2013;1066:89-101. https://doi.org/10.1007/978-1-62703-604-7_8

Bibtex

@article{8bda35621e534b988e3f184dd8050c00,
title = "In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature",
abstract = "In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.",
keywords = "Animals, Blood Platelets, Cell Adhesion, Cells, Cultured, Colonic Neoplasms, Endothelium, Vascular, Lung, Microcirculation, Microscopy, Fluorescence, Microscopy, Video, Microvessels, Neoplasm Metastasis, Rats, Rats, Sprague-Dawley",
author = "F Bartsch and Kang, {M L} and Mees, {S T} and J Haier and P Gassmann",
year = "2013",
doi = "10.1007/978-1-62703-604-7_8",
language = "English",
volume = "1066",
pages = "89--101",
journal = "Methods Mol Biol",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature

AU - Bartsch, F

AU - Kang, M L

AU - Mees, S T

AU - Haier, J

AU - Gassmann, P

PY - 2013

Y1 - 2013

N2 - In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.

AB - In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.

KW - Animals

KW - Blood Platelets

KW - Cell Adhesion

KW - Cells, Cultured

KW - Colonic Neoplasms

KW - Endothelium, Vascular

KW - Lung

KW - Microcirculation

KW - Microscopy, Fluorescence

KW - Microscopy, Video

KW - Microvessels

KW - Neoplasm Metastasis

KW - Rats

KW - Rats, Sprague-Dawley

U2 - 10.1007/978-1-62703-604-7_8

DO - 10.1007/978-1-62703-604-7_8

M3 - SCORING: Journal article

C2 - 23955736

VL - 1066

SP - 89

EP - 101

JO - Methods Mol Biol

JF - Methods Mol Biol

SN - 1064-3745

ER -