In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature
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In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature. / Bartsch, F; Kang, M L; Mees, S T; Haier, J; Gassmann, P.
in: Methods Mol Biol, Jahrgang 1066, 2013, S. 89-101.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature
AU - Bartsch, F
AU - Kang, M L
AU - Mees, S T
AU - Haier, J
AU - Gassmann, P
PY - 2013
Y1 - 2013
N2 - In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.
AB - In vivo and ex vivo fluorescence video microscopy used to be a well-established method in life science with a variety of applications, such as in inflammation or cancer research. In this book chapter, we describe a model of in vivo fluorescence microscopy of the rat's lung with the exclusive advantage of qualitative and quantitative in vivo analysis of cell adhesion within the complex microenvironment of the ventilated and perfused lung. Observation can include real-time, time-lapse, or fast-motion analysis. In our laboratory, we have used the model for qualitative and quantitative real-time analyses of metastatic colon cancer cell adhesion within the rat's pulmonary microcirculation. Using some modifications in another series, we have also applied the model to analyze thrombocyte and leucocyte adhesion within the pulmonary capillaries in experimental sepsis. For interventional studies, injected cells or animals may be pretreated with various reagents or drugs for further analysis of adhesion molecules involved in tumor cell-endothelial cell interactions.
KW - Animals
KW - Blood Platelets
KW - Cell Adhesion
KW - Cells, Cultured
KW - Colonic Neoplasms
KW - Endothelium, Vascular
KW - Lung
KW - Microcirculation
KW - Microscopy, Fluorescence
KW - Microscopy, Video
KW - Microvessels
KW - Neoplasm Metastasis
KW - Rats
KW - Rats, Sprague-Dawley
U2 - 10.1007/978-1-62703-604-7_8
DO - 10.1007/978-1-62703-604-7_8
M3 - SCORING: Journal article
C2 - 23955736
VL - 1066
SP - 89
EP - 101
JO - Methods Mol Biol
JF - Methods Mol Biol
SN - 1064-3745
ER -