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In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs

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In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs. / Bischofs, Esther; Lubs, Daniel; Fritzsche, Friederike; Meyer, Anne-Sophie; Bruckner, Thomas; Sohn, Christof; Eichbaum, Michael H R.

In: ANTICANCER RES, Vol. 32, No. 3, 03.2012, p. 767-771.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bischofs, E, Lubs, D, Fritzsche, F, Meyer, A-S, Bruckner, T, Sohn, C & Eichbaum, MHR 2012, 'In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs', ANTICANCER RES, vol. 32, no. 3, pp. 767-771.

APA

Bischofs, E., Lubs, D., Fritzsche, F., Meyer, A-S., Bruckner, T., Sohn, C., & Eichbaum, M. H. R. (2012). In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs. ANTICANCER RES, 32(3), 767-771.

Vancouver

Bischofs E, Lubs D, Fritzsche F, Meyer A-S, Bruckner T, Sohn C et al. In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs. ANTICANCER RES. 2012 Mar;32(3):767-771.

Bibtex

@article{7cf80336cd0c4876ab99844206963081,
title = "In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs",
abstract = "BACKGROUND: Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.MATERIALS AND METHODS: Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.RESULTS: Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.CONCLUSION: Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.",
keywords = "Anti-Inflammatory Agents, Non-Steroidal/pharmacology, Breast Neoplasms/pathology, Cell Adhesion/drug effects, Cell Line, Tumor, Endothelium, Vascular/drug effects, Female, Humans, In Vitro Techniques, Microscopy, Fluorescence",
author = "Esther Bischofs and Daniel Lubs and Friederike Fritzsche and Anne-Sophie Meyer and Thomas Bruckner and Christof Sohn and Eichbaum, {Michael H R}",
year = "2012",
month = mar,
language = "English",
volume = "32",
pages = "767--771",
journal = "ANTICANCER RES",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "3",

}

RIS

TY - JOUR

T1 - In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs

AU - Bischofs, Esther

AU - Lubs, Daniel

AU - Fritzsche, Friederike

AU - Meyer, Anne-Sophie

AU - Bruckner, Thomas

AU - Sohn, Christof

AU - Eichbaum, Michael H R

PY - 2012/3

Y1 - 2012/3

N2 - BACKGROUND: Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.MATERIALS AND METHODS: Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.RESULTS: Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.CONCLUSION: Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.

AB - BACKGROUND: Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.MATERIALS AND METHODS: Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.RESULTS: Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.CONCLUSION: Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.

KW - Anti-Inflammatory Agents, Non-Steroidal/pharmacology

KW - Breast Neoplasms/pathology

KW - Cell Adhesion/drug effects

KW - Cell Line, Tumor

KW - Endothelium, Vascular/drug effects

KW - Female

KW - Humans

KW - In Vitro Techniques

KW - Microscopy, Fluorescence

M3 - SCORING: Journal article

C2 - 22399590

VL - 32

SP - 767

EP - 771

JO - ANTICANCER RES

JF - ANTICANCER RES

SN - 0250-7005

IS - 3

ER -