In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs
Standard
In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs. / Bischofs, Esther; Lubs, Daniel; Fritzsche, Friederike; Meyer, Anne-Sophie; Bruckner, Thomas; Sohn, Christof; Eichbaum, Michael H R.
in: ANTICANCER RES, Jahrgang 32, Nr. 3, 03.2012, S. 767-771.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - In vitro blockade of adhesion of breast cancer cells to endothelial cells using anti-inflammatory drugs
AU - Bischofs, Esther
AU - Lubs, Daniel
AU - Fritzsche, Friederike
AU - Meyer, Anne-Sophie
AU - Bruckner, Thomas
AU - Sohn, Christof
AU - Eichbaum, Michael H R
PY - 2012/3
Y1 - 2012/3
N2 - BACKGROUND: Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.MATERIALS AND METHODS: Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.RESULTS: Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.CONCLUSION: Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.
AB - BACKGROUND: Increasing evidence suggests that a pro-inflammatory microenvironment affects distant metastasis of breast cancer cells, in particular by favoring tumor cell adhesion to endothelium. The aim of this study was to investigate the potential of different anti-inflammatory drugs to inhibit this effect in vitro.MATERIALS AND METHODS: Breast cancer cells from the metastatic cell line KM22 were incubated with activated Human umbilical vein endothelial cells (HUVECs). Tumor cell adhesion was quantified by fluorescence microscopy. The anti-inflammatory drugs ibuprofen, aspirin (acetylsalicylic acid), diclofenac, and dexamethasone were used as inhibiting agents.RESULTS: Aspirin and dexamethasone significantly reduced breast cancer cell adhesion to HUVECs (20.3%, p<0.000; and 25%, p<0.05, respectively). Ibuprofen and diclofenac did not significantly reduce tumor cell adhesion.CONCLUSION: Aspirin and dexamethasone seem to be able to partly inhibit adhesion of breast cancer cells to endothelium. Future studies should attempt to optimize this effect in vitro, in preparation for potential in vivo trials.
KW - Anti-Inflammatory Agents, Non-Steroidal/pharmacology
KW - Breast Neoplasms/pathology
KW - Cell Adhesion/drug effects
KW - Cell Line, Tumor
KW - Endothelium, Vascular/drug effects
KW - Female
KW - Humans
KW - In Vitro Techniques
KW - Microscopy, Fluorescence
M3 - SCORING: Journal article
C2 - 22399590
VL - 32
SP - 767
EP - 771
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 3
ER -