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In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering

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In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering. / Herrmann, Florian E M; Lehner, Anja; Hollweck, Trixi; Haas, Ulrike; Fano, Cornelia; Fehrenbach, David; Kozlik-Feldmann, Rainer; Wintermantel, Erich; Eissner, Gunther; Hagl, Christian; Akra, Bassil.

In: J BIOMED MATER RES A, Vol. 102, No. 4, 01.04.2014, p. 958-66.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Herrmann, FEM, Lehner, A, Hollweck, T, Haas, U, Fano, C, Fehrenbach, D, Kozlik-Feldmann, R, Wintermantel, E, Eissner, G, Hagl, C & Akra, B 2014, 'In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering', J BIOMED MATER RES A, vol. 102, no. 4, pp. 958-66. https://doi.org/10.1002/jbm.a.34786

APA

Herrmann, F. E. M., Lehner, A., Hollweck, T., Haas, U., Fano, C., Fehrenbach, D., Kozlik-Feldmann, R., Wintermantel, E., Eissner, G., Hagl, C., & Akra, B. (2014). In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering. J BIOMED MATER RES A, 102(4), 958-66. https://doi.org/10.1002/jbm.a.34786

Vancouver

Herrmann FEM, Lehner A, Hollweck T, Haas U, Fano C, Fehrenbach D et al. In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering. J BIOMED MATER RES A. 2014 Apr 1;102(4):958-66. https://doi.org/10.1002/jbm.a.34786

Bibtex

@article{5c25ca66d8c14e7dba71580f4ca84d58,
title = "In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering",
abstract = "A cardiac patch is a construct devised in regenerative medicine to replace necrotic heart tissue after myocardial infarctions. The cardiac patch consists of a scaffold seeded with stem cells. To identify the best scaffold for cardiac patch construction we compared polyurethane, Collagen Cell Carriers, ePTFE, and ePTFE SSP1-RGD regarding their receptiveness to seeding with mesenchymal stem cells isolated from umbilical cord tissue. Seeding was tested at an array of cell seeding densities. The bioartificial patches were cultured for up to 35 days and evaluated by scanning electron microscopy, microscopy of histological stains, fluorescence microscopy, and mitochondrial assays. Polyurethane was the only biomaterial which resulted in an organized multilayer (seeding density: 0.750 × 10(6) cells/cm(2)). Cultured over 35 days at this seeding density the mitochondrial activity of the cells on polyurethane patches continually increased. There was no decrease in the E Modulus of polyurethane once seeded with cells. Seeding of CCC could only be realized at a low seeding density and both ePTFE and ePTFE SSP1-RGD were found to be unreceptive to seeding. Of the tested scaffolds polyurethane thus crystallized as the most appropriate for seeding with mesenchymal stem cells in the framework of myocardial tissue engineering.",
keywords = "Biomechanical Phenomena, Collagen, Elastic Modulus, Humans, Materials Testing, Mesenchymal Stromal Cells, Microscopy, Electron, Scanning, Mitochondria, Myocardium, Polytetrafluoroethylene, Polyurethanes, Propidium, Staining and Labeling, Tissue Engineering, Tissue Scaffolds",
author = "Herrmann, {Florian E M} and Anja Lehner and Trixi Hollweck and Ulrike Haas and Cornelia Fano and David Fehrenbach and Rainer Kozlik-Feldmann and Erich Wintermantel and Gunther Eissner and Christian Hagl and Bassil Akra",
note = "Copyright {\textcopyright} 2013 Wiley Periodicals, Inc.",
year = "2014",
month = apr,
day = "1",
doi = "10.1002/jbm.a.34786",
language = "English",
volume = "102",
pages = "958--66",
journal = "J BIOMED MATER RES A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering

AU - Herrmann, Florian E M

AU - Lehner, Anja

AU - Hollweck, Trixi

AU - Haas, Ulrike

AU - Fano, Cornelia

AU - Fehrenbach, David

AU - Kozlik-Feldmann, Rainer

AU - Wintermantel, Erich

AU - Eissner, Gunther

AU - Hagl, Christian

AU - Akra, Bassil

N1 - Copyright © 2013 Wiley Periodicals, Inc.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - A cardiac patch is a construct devised in regenerative medicine to replace necrotic heart tissue after myocardial infarctions. The cardiac patch consists of a scaffold seeded with stem cells. To identify the best scaffold for cardiac patch construction we compared polyurethane, Collagen Cell Carriers, ePTFE, and ePTFE SSP1-RGD regarding their receptiveness to seeding with mesenchymal stem cells isolated from umbilical cord tissue. Seeding was tested at an array of cell seeding densities. The bioartificial patches were cultured for up to 35 days and evaluated by scanning electron microscopy, microscopy of histological stains, fluorescence microscopy, and mitochondrial assays. Polyurethane was the only biomaterial which resulted in an organized multilayer (seeding density: 0.750 × 10(6) cells/cm(2)). Cultured over 35 days at this seeding density the mitochondrial activity of the cells on polyurethane patches continually increased. There was no decrease in the E Modulus of polyurethane once seeded with cells. Seeding of CCC could only be realized at a low seeding density and both ePTFE and ePTFE SSP1-RGD were found to be unreceptive to seeding. Of the tested scaffolds polyurethane thus crystallized as the most appropriate for seeding with mesenchymal stem cells in the framework of myocardial tissue engineering.

AB - A cardiac patch is a construct devised in regenerative medicine to replace necrotic heart tissue after myocardial infarctions. The cardiac patch consists of a scaffold seeded with stem cells. To identify the best scaffold for cardiac patch construction we compared polyurethane, Collagen Cell Carriers, ePTFE, and ePTFE SSP1-RGD regarding their receptiveness to seeding with mesenchymal stem cells isolated from umbilical cord tissue. Seeding was tested at an array of cell seeding densities. The bioartificial patches were cultured for up to 35 days and evaluated by scanning electron microscopy, microscopy of histological stains, fluorescence microscopy, and mitochondrial assays. Polyurethane was the only biomaterial which resulted in an organized multilayer (seeding density: 0.750 × 10(6) cells/cm(2)). Cultured over 35 days at this seeding density the mitochondrial activity of the cells on polyurethane patches continually increased. There was no decrease in the E Modulus of polyurethane once seeded with cells. Seeding of CCC could only be realized at a low seeding density and both ePTFE and ePTFE SSP1-RGD were found to be unreceptive to seeding. Of the tested scaffolds polyurethane thus crystallized as the most appropriate for seeding with mesenchymal stem cells in the framework of myocardial tissue engineering.

KW - Biomechanical Phenomena

KW - Collagen

KW - Elastic Modulus

KW - Humans

KW - Materials Testing

KW - Mesenchymal Stromal Cells

KW - Microscopy, Electron, Scanning

KW - Mitochondria

KW - Myocardium

KW - Polytetrafluoroethylene

KW - Polyurethanes

KW - Propidium

KW - Staining and Labeling

KW - Tissue Engineering

KW - Tissue Scaffolds

U2 - 10.1002/jbm.a.34786

DO - 10.1002/jbm.a.34786

M3 - SCORING: Journal article

C2 - 23650018

VL - 102

SP - 958

EP - 966

JO - J BIOMED MATER RES A

JF - J BIOMED MATER RES A

SN - 1549-3296

IS - 4

ER -