In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering
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In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering. / Herrmann, Florian E M; Lehner, Anja; Hollweck, Trixi; Haas, Ulrike; Fano, Cornelia; Fehrenbach, David; Kozlik-Feldmann, Rainer; Wintermantel, Erich; Eissner, Gunther; Hagl, Christian; Akra, Bassil.
in: J BIOMED MATER RES A, Jahrgang 102, Nr. 4, 01.04.2014, S. 958-66.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vitro biological and mechanical evaluation of various scaffold materials for myocardial tissue engineering
AU - Herrmann, Florian E M
AU - Lehner, Anja
AU - Hollweck, Trixi
AU - Haas, Ulrike
AU - Fano, Cornelia
AU - Fehrenbach, David
AU - Kozlik-Feldmann, Rainer
AU - Wintermantel, Erich
AU - Eissner, Gunther
AU - Hagl, Christian
AU - Akra, Bassil
N1 - Copyright © 2013 Wiley Periodicals, Inc.
PY - 2014/4/1
Y1 - 2014/4/1
N2 - A cardiac patch is a construct devised in regenerative medicine to replace necrotic heart tissue after myocardial infarctions. The cardiac patch consists of a scaffold seeded with stem cells. To identify the best scaffold for cardiac patch construction we compared polyurethane, Collagen Cell Carriers, ePTFE, and ePTFE SSP1-RGD regarding their receptiveness to seeding with mesenchymal stem cells isolated from umbilical cord tissue. Seeding was tested at an array of cell seeding densities. The bioartificial patches were cultured for up to 35 days and evaluated by scanning electron microscopy, microscopy of histological stains, fluorescence microscopy, and mitochondrial assays. Polyurethane was the only biomaterial which resulted in an organized multilayer (seeding density: 0.750 × 10(6) cells/cm(2)). Cultured over 35 days at this seeding density the mitochondrial activity of the cells on polyurethane patches continually increased. There was no decrease in the E Modulus of polyurethane once seeded with cells. Seeding of CCC could only be realized at a low seeding density and both ePTFE and ePTFE SSP1-RGD were found to be unreceptive to seeding. Of the tested scaffolds polyurethane thus crystallized as the most appropriate for seeding with mesenchymal stem cells in the framework of myocardial tissue engineering.
AB - A cardiac patch is a construct devised in regenerative medicine to replace necrotic heart tissue after myocardial infarctions. The cardiac patch consists of a scaffold seeded with stem cells. To identify the best scaffold for cardiac patch construction we compared polyurethane, Collagen Cell Carriers, ePTFE, and ePTFE SSP1-RGD regarding their receptiveness to seeding with mesenchymal stem cells isolated from umbilical cord tissue. Seeding was tested at an array of cell seeding densities. The bioartificial patches were cultured for up to 35 days and evaluated by scanning electron microscopy, microscopy of histological stains, fluorescence microscopy, and mitochondrial assays. Polyurethane was the only biomaterial which resulted in an organized multilayer (seeding density: 0.750 × 10(6) cells/cm(2)). Cultured over 35 days at this seeding density the mitochondrial activity of the cells on polyurethane patches continually increased. There was no decrease in the E Modulus of polyurethane once seeded with cells. Seeding of CCC could only be realized at a low seeding density and both ePTFE and ePTFE SSP1-RGD were found to be unreceptive to seeding. Of the tested scaffolds polyurethane thus crystallized as the most appropriate for seeding with mesenchymal stem cells in the framework of myocardial tissue engineering.
KW - Biomechanical Phenomena
KW - Collagen
KW - Elastic Modulus
KW - Humans
KW - Materials Testing
KW - Mesenchymal Stromal Cells
KW - Microscopy, Electron, Scanning
KW - Mitochondria
KW - Myocardium
KW - Polytetrafluoroethylene
KW - Polyurethanes
KW - Propidium
KW - Staining and Labeling
KW - Tissue Engineering
KW - Tissue Scaffolds
U2 - 10.1002/jbm.a.34786
DO - 10.1002/jbm.a.34786
M3 - SCORING: Journal article
C2 - 23650018
VL - 102
SP - 958
EP - 966
JO - J BIOMED MATER RES A
JF - J BIOMED MATER RES A
SN - 1549-3296
IS - 4
ER -