In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts
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In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts. / Bakopoulou, Athina A; Triviai, Ioanna N; Tsiftsoglou, Asterios S; Garefis, Paylos D.
In: INT J PROSTHODONT, Vol. 19, No. 1, 17.02.2006, p. 13-6.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts
AU - Bakopoulou, Athina A
AU - Triviai, Ioanna N
AU - Tsiftsoglou, Asterios S
AU - Garefis, Paylos D
PY - 2006/2/17
Y1 - 2006/2/17
N2 - PURPOSE: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.MATERIALS AND METHODS: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.RESULTS: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.CONCLUSION: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.
AB - PURPOSE: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.MATERIALS AND METHODS: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.RESULTS: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.CONCLUSION: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.
KW - Animals
KW - Cell Count
KW - Cell Line
KW - Cell Survival
KW - Ceramics
KW - Coloring Agents
KW - Composite Resins
KW - DNA Damage
KW - Dental Materials
KW - Fibroblasts
KW - Glass Ionomer Cements
KW - Materials Testing
KW - Mutagens
KW - Silicate Cement
KW - Time Factors
KW - Trypan Blue
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - SCORING: Journal article
C2 - 16479751
VL - 19
SP - 13
EP - 16
JO - INT J PROSTHODONT
JF - INT J PROSTHODONT
SN - 0893-2174
IS - 1
ER -