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In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts

Standard

In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts. / Bakopoulou, Athina A; Triviai, Ioanna N; Tsiftsoglou, Asterios S; Garefis, Paylos D.

in: INT J PROSTHODONT, Jahrgang 19, Nr. 1, 17.02.2006, S. 13-6.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Bakopoulou, AA, Triviai, IN, Tsiftsoglou, AS & Garefis, PD 2006, 'In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts', INT J PROSTHODONT, Jg. 19, Nr. 1, S. 13-6.

APA

Bakopoulou, A. A., Triviai, I. N., Tsiftsoglou, A. S., & Garefis, P. D. (2006). In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts. INT J PROSTHODONT, 19(1), 13-6.

Vancouver

Bakopoulou AA, Triviai IN, Tsiftsoglou AS, Garefis PD. In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts. INT J PROSTHODONT. 2006 Feb 17;19(1):13-6.

Bibtex

@article{eba6956a4bb54dd9b245f6da09ebbc64,
title = "In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts",
abstract = "PURPOSE: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.MATERIALS AND METHODS: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.RESULTS: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.CONCLUSION: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.",
keywords = "Animals, Cell Count, Cell Line, Cell Survival, Ceramics, Coloring Agents, Composite Resins, DNA Damage, Dental Materials, Fibroblasts, Glass Ionomer Cements, Materials Testing, Mutagens, Silicate Cement, Time Factors, Trypan Blue, Journal Article, Research Support, Non-U.S. Gov't",
author = "Bakopoulou, {Athina A} and Triviai, {Ioanna N} and Tsiftsoglou, {Asterios S} and Garefis, {Paylos D}",
year = "2006",
month = feb,
day = "17",
language = "English",
volume = "19",
pages = "13--6",
journal = "INT J PROSTHODONT",
issn = "0893-2174",
publisher = "Quintessence Publishing Company",
number = "1",

}

RIS

TY - JOUR

T1 - In vitro assessment of cytotoxicity of resin-based dental restorative materials on WEHI 13 var fibroblasts

AU - Bakopoulou, Athina A

AU - Triviai, Ioanna N

AU - Tsiftsoglou, Asterios S

AU - Garefis, Paylos D

PY - 2006/2/17

Y1 - 2006/2/17

N2 - PURPOSE: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.MATERIALS AND METHODS: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.RESULTS: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.CONCLUSION: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.

AB - PURPOSE: The aim of this study was to assess the cytotoxicity of 4 resin composites' eluates on WEHI 13 Var fibroblasts as they aged in a biologic medium.MATERIALS AND METHODS: Cytotoxicity was determined by counting the number of viable cells by trypan blue exclusion. Morphologic changes attributable to cytotoxicity were observed by May-Grunwald-Giemsa cytologic staining and microscopic examination. DNA gel electrophoresis was performed to detect possible genotoxicity and DNA damage.RESULTS: All resin composite eluates, except that for Targis, caused a pronounced cytotoxicity during the first 72 hours that gradually decreased after 2 weeks of aging. Severe morphologic alterations and pronounced DNA damage were also observed.CONCLUSION: These findings indicate that resin-based dental restorative materials release agents cytotoxic and genotoxic to fibroblasts. Cytotoxicity is gradually decreased as the composite resins age in a biologic-relevant medium.

KW - Animals

KW - Cell Count

KW - Cell Line

KW - Cell Survival

KW - Ceramics

KW - Coloring Agents

KW - Composite Resins

KW - DNA Damage

KW - Dental Materials

KW - Fibroblasts

KW - Glass Ionomer Cements

KW - Materials Testing

KW - Mutagens

KW - Silicate Cement

KW - Time Factors

KW - Trypan Blue

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 16479751

VL - 19

SP - 13

EP - 16

JO - INT J PROSTHODONT

JF - INT J PROSTHODONT

SN - 0893-2174

IS - 1

ER -