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In vitro activity of photoactivated disinfection using a diode laser in infected root canals

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In vitro activity of photoactivated disinfection using a diode laser in infected root canals. / Schiffner, Ulrich; Cachovan, Georg; Bastian, Jochen; Sculean, Anton; Eick, Sigrun.

In: ACTA ODONTOL SCAND, Vol. 72, No. 8, 11.2014, p. 673-80.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schiffner, U, Cachovan, G, Bastian, J, Sculean, A & Eick, S 2014, 'In vitro activity of photoactivated disinfection using a diode laser in infected root canals', ACTA ODONTOL SCAND, vol. 72, no. 8, pp. 673-80. https://doi.org/10.3109/00016357.2014.898087

APA

Schiffner, U., Cachovan, G., Bastian, J., Sculean, A., & Eick, S. (2014). In vitro activity of photoactivated disinfection using a diode laser in infected root canals. ACTA ODONTOL SCAND, 72(8), 673-80. https://doi.org/10.3109/00016357.2014.898087

Vancouver

Schiffner U, Cachovan G, Bastian J, Sculean A, Eick S. In vitro activity of photoactivated disinfection using a diode laser in infected root canals. ACTA ODONTOL SCAND. 2014 Nov;72(8):673-80. https://doi.org/10.3109/00016357.2014.898087

Bibtex

@article{3dc4e6b7199c4d4e8bcf3911c4a23e42,
title = "In vitro activity of photoactivated disinfection using a diode laser in infected root canals",
abstract = "OBJECTIVE: To investigate the lethal activity of photoactivated disinfection (PAD) on Enterococcus faecalis (ATCC 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (PS).MATERIALS AND METHODS: First, the bactericidal activity of a low power diode laser (200 mW) against E. faecalis ATCC 29212 pre-treated with a PS (toluidine blue) for 2 min were examined after different irradiation times (30 s, 60 s and 90 s). The bactericidal activity in the presence of human serum or human serum albumin (HSA) was also examined. Second, root canals were infected with E. faecalis or with mixed aerobic or anaerobic microbial populations for 3 days and then irrigated with 1.5% sodium hypochlorite and exposed to PAD for 60 s.RESULTS: Photosensitization followed by laser irradiation for 60 s was sufficient to kill E. faecalis. Bacteria suspended in human serum (25% v/v) were totally eradicated after 30 s of irradiation. The addition of HSA (25 mg/ml or 50 mg/ml) to bacterial suspensions increased the antimicrobial efficacy of PAD after an irradiation time of 30 s, but no longer. The bactericidal effect of sodium hypochlorite was only enhanced by PAD during the early stages of treatment. PAD did not enhance the activity of sodium hypochlorite against a mixture of anaerobic bacteria.CONCLUSIONS: The bactericidal activity of PAD appears to be enhanced by serum proteins in vitro, but is limited to bacteria present within the root canal.",
keywords = "Actinobacteria, Actinomyces, Bacteremia, Bacterial Load, Bifidobacterium, Blood Bactericidal Activity, Dental Pulp Cavity, Disinfection, Enterococcus faecalis, Humans, Lasers, Semiconductor, Low-Level Light Therapy, Materials Testing, Peptostreptococcus, Photochemotherapy, Photosensitizing Agents, Root Canal Irrigants, Serum Albumin, Shewanella, Sodium Hypochlorite, Time Factors, Tolonium Chloride, Journal Article, Research Support, Non-U.S. Gov't",
author = "Ulrich Schiffner and Georg Cachovan and Jochen Bastian and Anton Sculean and Sigrun Eick",
year = "2014",
month = nov,
doi = "10.3109/00016357.2014.898087",
language = "English",
volume = "72",
pages = "673--80",
journal = "ACTA ODONTOL SCAND",
issn = "0001-6357",
publisher = "informa healthcare",
number = "8",

}

RIS

TY - JOUR

T1 - In vitro activity of photoactivated disinfection using a diode laser in infected root canals

AU - Schiffner, Ulrich

AU - Cachovan, Georg

AU - Bastian, Jochen

AU - Sculean, Anton

AU - Eick, Sigrun

PY - 2014/11

Y1 - 2014/11

N2 - OBJECTIVE: To investigate the lethal activity of photoactivated disinfection (PAD) on Enterococcus faecalis (ATCC 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (PS).MATERIALS AND METHODS: First, the bactericidal activity of a low power diode laser (200 mW) against E. faecalis ATCC 29212 pre-treated with a PS (toluidine blue) for 2 min were examined after different irradiation times (30 s, 60 s and 90 s). The bactericidal activity in the presence of human serum or human serum albumin (HSA) was also examined. Second, root canals were infected with E. faecalis or with mixed aerobic or anaerobic microbial populations for 3 days and then irrigated with 1.5% sodium hypochlorite and exposed to PAD for 60 s.RESULTS: Photosensitization followed by laser irradiation for 60 s was sufficient to kill E. faecalis. Bacteria suspended in human serum (25% v/v) were totally eradicated after 30 s of irradiation. The addition of HSA (25 mg/ml or 50 mg/ml) to bacterial suspensions increased the antimicrobial efficacy of PAD after an irradiation time of 30 s, but no longer. The bactericidal effect of sodium hypochlorite was only enhanced by PAD during the early stages of treatment. PAD did not enhance the activity of sodium hypochlorite against a mixture of anaerobic bacteria.CONCLUSIONS: The bactericidal activity of PAD appears to be enhanced by serum proteins in vitro, but is limited to bacteria present within the root canal.

AB - OBJECTIVE: To investigate the lethal activity of photoactivated disinfection (PAD) on Enterococcus faecalis (ATCC 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (PS).MATERIALS AND METHODS: First, the bactericidal activity of a low power diode laser (200 mW) against E. faecalis ATCC 29212 pre-treated with a PS (toluidine blue) for 2 min were examined after different irradiation times (30 s, 60 s and 90 s). The bactericidal activity in the presence of human serum or human serum albumin (HSA) was also examined. Second, root canals were infected with E. faecalis or with mixed aerobic or anaerobic microbial populations for 3 days and then irrigated with 1.5% sodium hypochlorite and exposed to PAD for 60 s.RESULTS: Photosensitization followed by laser irradiation for 60 s was sufficient to kill E. faecalis. Bacteria suspended in human serum (25% v/v) were totally eradicated after 30 s of irradiation. The addition of HSA (25 mg/ml or 50 mg/ml) to bacterial suspensions increased the antimicrobial efficacy of PAD after an irradiation time of 30 s, but no longer. The bactericidal effect of sodium hypochlorite was only enhanced by PAD during the early stages of treatment. PAD did not enhance the activity of sodium hypochlorite against a mixture of anaerobic bacteria.CONCLUSIONS: The bactericidal activity of PAD appears to be enhanced by serum proteins in vitro, but is limited to bacteria present within the root canal.

KW - Actinobacteria

KW - Actinomyces

KW - Bacteremia

KW - Bacterial Load

KW - Bifidobacterium

KW - Blood Bactericidal Activity

KW - Dental Pulp Cavity

KW - Disinfection

KW - Enterococcus faecalis

KW - Humans

KW - Lasers, Semiconductor

KW - Low-Level Light Therapy

KW - Materials Testing

KW - Peptostreptococcus

KW - Photochemotherapy

KW - Photosensitizing Agents

KW - Root Canal Irrigants

KW - Serum Albumin

KW - Shewanella

KW - Sodium Hypochlorite

KW - Time Factors

KW - Tolonium Chloride

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.3109/00016357.2014.898087

DO - 10.3109/00016357.2014.898087

M3 - SCORING: Journal article

C2 - 24655313

VL - 72

SP - 673

EP - 680

JO - ACTA ODONTOL SCAND

JF - ACTA ODONTOL SCAND

SN - 0001-6357

IS - 8

ER -