In vitro activity of photoactivated disinfection using a diode laser in infected root canals
Standard
In vitro activity of photoactivated disinfection using a diode laser in infected root canals. / Schiffner, Ulrich; Cachovan, Georg; Bastian, Jochen; Sculean, Anton; Eick, Sigrun.
in: ACTA ODONTOL SCAND, Jahrgang 72, Nr. 8, 11.2014, S. 673-80.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - In vitro activity of photoactivated disinfection using a diode laser in infected root canals
AU - Schiffner, Ulrich
AU - Cachovan, Georg
AU - Bastian, Jochen
AU - Sculean, Anton
AU - Eick, Sigrun
PY - 2014/11
Y1 - 2014/11
N2 - OBJECTIVE: To investigate the lethal activity of photoactivated disinfection (PAD) on Enterococcus faecalis (ATCC 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (PS).MATERIALS AND METHODS: First, the bactericidal activity of a low power diode laser (200 mW) against E. faecalis ATCC 29212 pre-treated with a PS (toluidine blue) for 2 min were examined after different irradiation times (30 s, 60 s and 90 s). The bactericidal activity in the presence of human serum or human serum albumin (HSA) was also examined. Second, root canals were infected with E. faecalis or with mixed aerobic or anaerobic microbial populations for 3 days and then irrigated with 1.5% sodium hypochlorite and exposed to PAD for 60 s.RESULTS: Photosensitization followed by laser irradiation for 60 s was sufficient to kill E. faecalis. Bacteria suspended in human serum (25% v/v) were totally eradicated after 30 s of irradiation. The addition of HSA (25 mg/ml or 50 mg/ml) to bacterial suspensions increased the antimicrobial efficacy of PAD after an irradiation time of 30 s, but no longer. The bactericidal effect of sodium hypochlorite was only enhanced by PAD during the early stages of treatment. PAD did not enhance the activity of sodium hypochlorite against a mixture of anaerobic bacteria.CONCLUSIONS: The bactericidal activity of PAD appears to be enhanced by serum proteins in vitro, but is limited to bacteria present within the root canal.
AB - OBJECTIVE: To investigate the lethal activity of photoactivated disinfection (PAD) on Enterococcus faecalis (ATCC 29212) and mixed populations of aerobic or anaerobic bacteria in infected root canals using a diode laser after the application of a photosensitizer (PS).MATERIALS AND METHODS: First, the bactericidal activity of a low power diode laser (200 mW) against E. faecalis ATCC 29212 pre-treated with a PS (toluidine blue) for 2 min were examined after different irradiation times (30 s, 60 s and 90 s). The bactericidal activity in the presence of human serum or human serum albumin (HSA) was also examined. Second, root canals were infected with E. faecalis or with mixed aerobic or anaerobic microbial populations for 3 days and then irrigated with 1.5% sodium hypochlorite and exposed to PAD for 60 s.RESULTS: Photosensitization followed by laser irradiation for 60 s was sufficient to kill E. faecalis. Bacteria suspended in human serum (25% v/v) were totally eradicated after 30 s of irradiation. The addition of HSA (25 mg/ml or 50 mg/ml) to bacterial suspensions increased the antimicrobial efficacy of PAD after an irradiation time of 30 s, but no longer. The bactericidal effect of sodium hypochlorite was only enhanced by PAD during the early stages of treatment. PAD did not enhance the activity of sodium hypochlorite against a mixture of anaerobic bacteria.CONCLUSIONS: The bactericidal activity of PAD appears to be enhanced by serum proteins in vitro, but is limited to bacteria present within the root canal.
KW - Actinobacteria
KW - Actinomyces
KW - Bacteremia
KW - Bacterial Load
KW - Bifidobacterium
KW - Blood Bactericidal Activity
KW - Dental Pulp Cavity
KW - Disinfection
KW - Enterococcus faecalis
KW - Humans
KW - Lasers, Semiconductor
KW - Low-Level Light Therapy
KW - Materials Testing
KW - Peptostreptococcus
KW - Photochemotherapy
KW - Photosensitizing Agents
KW - Root Canal Irrigants
KW - Serum Albumin
KW - Shewanella
KW - Sodium Hypochlorite
KW - Time Factors
KW - Tolonium Chloride
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.3109/00016357.2014.898087
DO - 10.3109/00016357.2014.898087
M3 - SCORING: Journal article
C2 - 24655313
VL - 72
SP - 673
EP - 680
JO - ACTA ODONTOL SCAND
JF - ACTA ODONTOL SCAND
SN - 0001-6357
IS - 8
ER -