Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche
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Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. / Kloepper, Jennifer Elisabeth; Tiede, Stephan; Brinckmann, Jürgen; Reinhardt, Dieter Peter; Meyer, Wilfried; Faessler, Reinhard; Paus, Ralf.
In: EXP DERMATOL, Vol. 17, No. 7, 07.2008, p. 592-609.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche
AU - Kloepper, Jennifer Elisabeth
AU - Tiede, Stephan
AU - Brinckmann, Jürgen
AU - Reinhardt, Dieter Peter
AU - Meyer, Wilfried
AU - Faessler, Reinhard
AU - Paus, Ralf
PY - 2008/7
Y1 - 2008/7
N2 - Since the discovery of epithelial hair follicle stem cells (eHFSCs) in the bulge of human hair follicles (HFs) an important quest has started: to define useful markers. In the current study, we contribute to this by critically evaluating corresponding published immunoreactivity (IR) patterns, and by attempting to identify markers for the in situ identification of human eHFSCs and their niche. For this, human scalp skin cryosections of at least five different individuals were examined, employing standard immunohistology as well as increased sensitivity methods. Defined reference areas were compared by quantitative immunohistochemistry for the relative intensity of their specific IR. According to our experience, the most useful positive markers for human bulge cells turned out to be cytokeratin 15, cytokeratin 19 and CD200, but were not exclusive, while beta1 integrin and Lhx2 IR were not upregulated by human bulge keratinocytes. Absent IR for CD34, connexin43 and nestin on human bulge cells may be exploited as negative markers. alpha6 integrin, fibronectin, nidogen, fibrillin-1 and latent transforming growth factor (TGF)-beta-binding protein-1 were expressed throughout the connective tissue sheath of human HFs. On the other hand, tenascin-C was upregulated in the bulge and may thus constitute a component of the bulge stem cell niche of human HFs. These immunophenotyping results shed further light on the in situ expression patterns of claimed follicular 'stem cell markers' and suggest that not a single marker alone but only the use of a limited corresponding panel of positive and negative markers may offer a reasonable and pragmatic compromise for identifying human bulge stem cells in situ.
AB - Since the discovery of epithelial hair follicle stem cells (eHFSCs) in the bulge of human hair follicles (HFs) an important quest has started: to define useful markers. In the current study, we contribute to this by critically evaluating corresponding published immunoreactivity (IR) patterns, and by attempting to identify markers for the in situ identification of human eHFSCs and their niche. For this, human scalp skin cryosections of at least five different individuals were examined, employing standard immunohistology as well as increased sensitivity methods. Defined reference areas were compared by quantitative immunohistochemistry for the relative intensity of their specific IR. According to our experience, the most useful positive markers for human bulge cells turned out to be cytokeratin 15, cytokeratin 19 and CD200, but were not exclusive, while beta1 integrin and Lhx2 IR were not upregulated by human bulge keratinocytes. Absent IR for CD34, connexin43 and nestin on human bulge cells may be exploited as negative markers. alpha6 integrin, fibronectin, nidogen, fibrillin-1 and latent transforming growth factor (TGF)-beta-binding protein-1 were expressed throughout the connective tissue sheath of human HFs. On the other hand, tenascin-C was upregulated in the bulge and may thus constitute a component of the bulge stem cell niche of human HFs. These immunophenotyping results shed further light on the in situ expression patterns of claimed follicular 'stem cell markers' and suggest that not a single marker alone but only the use of a limited corresponding panel of positive and negative markers may offer a reasonable and pragmatic compromise for identifying human bulge stem cells in situ.
KW - Adult Stem Cells
KW - Antigens, CD
KW - Antigens, CD34
KW - Biomarkers
KW - Connexin 43
KW - Fibrillin-1
KW - Fibrillins
KW - Fluorescent Antibody Technique
KW - Hair Follicle
KW - Homeodomain Proteins
KW - Humans
KW - Immunohistochemistry
KW - Immunophenotyping
KW - Intermediate Filament Proteins
KW - Keratin-15
KW - Keratin-19
KW - LIM-Homeodomain Proteins
KW - Membrane Proteins
KW - Microfilament Proteins
KW - Microscopy, Fluorescence
KW - Nerve Tissue Proteins
KW - Nestin
KW - Statistics, Nonparametric
KW - Tenascin
KW - Transcription Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/j.1600-0625.2008.00720.x
DO - 10.1111/j.1600-0625.2008.00720.x
M3 - SCORING: Journal article
C2 - 18558994
VL - 17
SP - 592
EP - 609
JO - EXP DERMATOL
JF - EXP DERMATOL
SN - 0906-6705
IS - 7
ER -