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Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche

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Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. / Kloepper, Jennifer Elisabeth; Tiede, Stephan; Brinckmann, Jürgen; Reinhardt, Dieter Peter; Meyer, Wilfried; Faessler, Reinhard; Paus, Ralf.

in: EXP DERMATOL, Jahrgang 17, Nr. 7, 07.2008, S. 592-609.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kloepper, JE, Tiede, S, Brinckmann, J, Reinhardt, DP, Meyer, W, Faessler, R & Paus, R 2008, 'Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche', EXP DERMATOL, Jg. 17, Nr. 7, S. 592-609. https://doi.org/10.1111/j.1600-0625.2008.00720.x

APA

Kloepper, J. E., Tiede, S., Brinckmann, J., Reinhardt, D. P., Meyer, W., Faessler, R., & Paus, R. (2008). Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. EXP DERMATOL, 17(7), 592-609. https://doi.org/10.1111/j.1600-0625.2008.00720.x

Vancouver

Kloepper JE, Tiede S, Brinckmann J, Reinhardt DP, Meyer W, Faessler R et al. Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche. EXP DERMATOL. 2008 Jul;17(7):592-609. https://doi.org/10.1111/j.1600-0625.2008.00720.x

Bibtex

@article{bbaa8b41ed37499fba2f2a5ea0fa7c8a,
title = "Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche",
abstract = "Since the discovery of epithelial hair follicle stem cells (eHFSCs) in the bulge of human hair follicles (HFs) an important quest has started: to define useful markers. In the current study, we contribute to this by critically evaluating corresponding published immunoreactivity (IR) patterns, and by attempting to identify markers for the in situ identification of human eHFSCs and their niche. For this, human scalp skin cryosections of at least five different individuals were examined, employing standard immunohistology as well as increased sensitivity methods. Defined reference areas were compared by quantitative immunohistochemistry for the relative intensity of their specific IR. According to our experience, the most useful positive markers for human bulge cells turned out to be cytokeratin 15, cytokeratin 19 and CD200, but were not exclusive, while beta1 integrin and Lhx2 IR were not upregulated by human bulge keratinocytes. Absent IR for CD34, connexin43 and nestin on human bulge cells may be exploited as negative markers. alpha6 integrin, fibronectin, nidogen, fibrillin-1 and latent transforming growth factor (TGF)-beta-binding protein-1 were expressed throughout the connective tissue sheath of human HFs. On the other hand, tenascin-C was upregulated in the bulge and may thus constitute a component of the bulge stem cell niche of human HFs. These immunophenotyping results shed further light on the in situ expression patterns of claimed follicular 'stem cell markers' and suggest that not a single marker alone but only the use of a limited corresponding panel of positive and negative markers may offer a reasonable and pragmatic compromise for identifying human bulge stem cells in situ.",
keywords = "Adult Stem Cells, Antigens, CD, Antigens, CD34, Biomarkers, Connexin 43, Fibrillin-1, Fibrillins, Fluorescent Antibody Technique, Hair Follicle, Homeodomain Proteins, Humans, Immunohistochemistry, Immunophenotyping, Intermediate Filament Proteins, Keratin-15, Keratin-19, LIM-Homeodomain Proteins, Membrane Proteins, Microfilament Proteins, Microscopy, Fluorescence, Nerve Tissue Proteins, Nestin, Statistics, Nonparametric, Tenascin, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't",
author = "Kloepper, {Jennifer Elisabeth} and Stephan Tiede and J{\"u}rgen Brinckmann and Reinhardt, {Dieter Peter} and Wilfried Meyer and Reinhard Faessler and Ralf Paus",
year = "2008",
month = jul,
doi = "10.1111/j.1600-0625.2008.00720.x",
language = "English",
volume = "17",
pages = "592--609",
journal = "EXP DERMATOL",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "7",

}

RIS

TY - JOUR

T1 - Immunophenotyping of the human bulge Region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche

AU - Kloepper, Jennifer Elisabeth

AU - Tiede, Stephan

AU - Brinckmann, Jürgen

AU - Reinhardt, Dieter Peter

AU - Meyer, Wilfried

AU - Faessler, Reinhard

AU - Paus, Ralf

PY - 2008/7

Y1 - 2008/7

N2 - Since the discovery of epithelial hair follicle stem cells (eHFSCs) in the bulge of human hair follicles (HFs) an important quest has started: to define useful markers. In the current study, we contribute to this by critically evaluating corresponding published immunoreactivity (IR) patterns, and by attempting to identify markers for the in situ identification of human eHFSCs and their niche. For this, human scalp skin cryosections of at least five different individuals were examined, employing standard immunohistology as well as increased sensitivity methods. Defined reference areas were compared by quantitative immunohistochemistry for the relative intensity of their specific IR. According to our experience, the most useful positive markers for human bulge cells turned out to be cytokeratin 15, cytokeratin 19 and CD200, but were not exclusive, while beta1 integrin and Lhx2 IR were not upregulated by human bulge keratinocytes. Absent IR for CD34, connexin43 and nestin on human bulge cells may be exploited as negative markers. alpha6 integrin, fibronectin, nidogen, fibrillin-1 and latent transforming growth factor (TGF)-beta-binding protein-1 were expressed throughout the connective tissue sheath of human HFs. On the other hand, tenascin-C was upregulated in the bulge and may thus constitute a component of the bulge stem cell niche of human HFs. These immunophenotyping results shed further light on the in situ expression patterns of claimed follicular 'stem cell markers' and suggest that not a single marker alone but only the use of a limited corresponding panel of positive and negative markers may offer a reasonable and pragmatic compromise for identifying human bulge stem cells in situ.

AB - Since the discovery of epithelial hair follicle stem cells (eHFSCs) in the bulge of human hair follicles (HFs) an important quest has started: to define useful markers. In the current study, we contribute to this by critically evaluating corresponding published immunoreactivity (IR) patterns, and by attempting to identify markers for the in situ identification of human eHFSCs and their niche. For this, human scalp skin cryosections of at least five different individuals were examined, employing standard immunohistology as well as increased sensitivity methods. Defined reference areas were compared by quantitative immunohistochemistry for the relative intensity of their specific IR. According to our experience, the most useful positive markers for human bulge cells turned out to be cytokeratin 15, cytokeratin 19 and CD200, but were not exclusive, while beta1 integrin and Lhx2 IR were not upregulated by human bulge keratinocytes. Absent IR for CD34, connexin43 and nestin on human bulge cells may be exploited as negative markers. alpha6 integrin, fibronectin, nidogen, fibrillin-1 and latent transforming growth factor (TGF)-beta-binding protein-1 were expressed throughout the connective tissue sheath of human HFs. On the other hand, tenascin-C was upregulated in the bulge and may thus constitute a component of the bulge stem cell niche of human HFs. These immunophenotyping results shed further light on the in situ expression patterns of claimed follicular 'stem cell markers' and suggest that not a single marker alone but only the use of a limited corresponding panel of positive and negative markers may offer a reasonable and pragmatic compromise for identifying human bulge stem cells in situ.

KW - Adult Stem Cells

KW - Antigens, CD

KW - Antigens, CD34

KW - Biomarkers

KW - Connexin 43

KW - Fibrillin-1

KW - Fibrillins

KW - Fluorescent Antibody Technique

KW - Hair Follicle

KW - Homeodomain Proteins

KW - Humans

KW - Immunohistochemistry

KW - Immunophenotyping

KW - Intermediate Filament Proteins

KW - Keratin-15

KW - Keratin-19

KW - LIM-Homeodomain Proteins

KW - Membrane Proteins

KW - Microfilament Proteins

KW - Microscopy, Fluorescence

KW - Nerve Tissue Proteins

KW - Nestin

KW - Statistics, Nonparametric

KW - Tenascin

KW - Transcription Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1111/j.1600-0625.2008.00720.x

DO - 10.1111/j.1600-0625.2008.00720.x

M3 - SCORING: Journal article

C2 - 18558994

VL - 17

SP - 592

EP - 609

JO - EXP DERMATOL

JF - EXP DERMATOL

SN - 0906-6705

IS - 7

ER -