Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia
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Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia. / Schultheiss, Maximilian; Schnichels, Sven; Hermann, Thoralf; Hurst, Jose; Feldkaemper, Marita; Arango-Gonzalez, Blanca; Ueffing, Marius; Bartz-Schmidt, Karl U; Zeck, Guenther; Spitzer, Martin S.
In: PLOS ONE, Vol. 11, No. 2, 2016, p. e0148616.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia
AU - Schultheiss, Maximilian
AU - Schnichels, Sven
AU - Hermann, Thoralf
AU - Hurst, Jose
AU - Feldkaemper, Marita
AU - Arango-Gonzalez, Blanca
AU - Ueffing, Marius
AU - Bartz-Schmidt, Karl U
AU - Zeck, Guenther
AU - Spitzer, Martin S
PY - 2016
Y1 - 2016
N2 - PURPOSE: Hypothermia has been shown to be neuroprotective in the therapy of ischemic stroke in the brain. To date no studies exist on the level of the inner retina and it is unclear if hypothermia would prolong the ischemic tolerance time of retinal ganglion cells, which are decisive in many ischemic retinopathies.METHODS: Bovine eyes were enucleated and stored either at 21°C or 37°C for 100 or 340 minutes, respectively. Afterwards the globes were dissected, the retina was prepared and either the spontaneous ganglion cell responses were measured or the retina was incubated as an organotypic culture for additional 24 hours. After incubation the retina was either processed for histology (H&E and DAPI staining) or real-time PCR (Thy-1 expression) was performed.RESULTS: Hypothermia prolonged ganglion cell survival up to 340 minutes under ischemic conditions. In contrast to eyes kept at 37°C the eyes stored at 21°C still showed spontaneous ganglion cell spiking (56.8% versus 0%), a 5.8 fold higher Thy-1 mRNA expression (not significant, but a trend) and a preserved retinal structure after 340 minutes of ischemia.CONCLUSION: Hypothermia protects retinal ganglion cells against ischemia and prolongs their ischemic tolerance time.
AB - PURPOSE: Hypothermia has been shown to be neuroprotective in the therapy of ischemic stroke in the brain. To date no studies exist on the level of the inner retina and it is unclear if hypothermia would prolong the ischemic tolerance time of retinal ganglion cells, which are decisive in many ischemic retinopathies.METHODS: Bovine eyes were enucleated and stored either at 21°C or 37°C for 100 or 340 minutes, respectively. Afterwards the globes were dissected, the retina was prepared and either the spontaneous ganglion cell responses were measured or the retina was incubated as an organotypic culture for additional 24 hours. After incubation the retina was either processed for histology (H&E and DAPI staining) or real-time PCR (Thy-1 expression) was performed.RESULTS: Hypothermia prolonged ganglion cell survival up to 340 minutes under ischemic conditions. In contrast to eyes kept at 37°C the eyes stored at 21°C still showed spontaneous ganglion cell spiking (56.8% versus 0%), a 5.8 fold higher Thy-1 mRNA expression (not significant, but a trend) and a preserved retinal structure after 340 minutes of ischemia.CONCLUSION: Hypothermia protects retinal ganglion cells against ischemia and prolongs their ischemic tolerance time.
KW - Animals
KW - Antigens, Thy-1
KW - Cattle
KW - Cold Temperature
KW - Eye
KW - Hypothermia, Induced
KW - Ischemia
KW - Retinal Ganglion Cells
KW - Time Factors
KW - Journal Article
U2 - 10.1371/journal.pone.0148616
DO - 10.1371/journal.pone.0148616
M3 - SCORING: Journal article
C2 - 26848953
VL - 11
SP - e0148616
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 2
ER -