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Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia

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Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia. / Schultheiss, Maximilian; Schnichels, Sven; Hermann, Thoralf; Hurst, Jose; Feldkaemper, Marita; Arango-Gonzalez, Blanca; Ueffing, Marius; Bartz-Schmidt, Karl U; Zeck, Guenther; Spitzer, Martin S.

in: PLOS ONE, Jahrgang 11, Nr. 2, 2016, S. e0148616.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Schultheiss, M, Schnichels, S, Hermann, T, Hurst, J, Feldkaemper, M, Arango-Gonzalez, B, Ueffing, M, Bartz-Schmidt, KU, Zeck, G & Spitzer, MS 2016, 'Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia', PLOS ONE, Jg. 11, Nr. 2, S. e0148616. https://doi.org/10.1371/journal.pone.0148616

APA

Schultheiss, M., Schnichels, S., Hermann, T., Hurst, J., Feldkaemper, M., Arango-Gonzalez, B., Ueffing, M., Bartz-Schmidt, K. U., Zeck, G., & Spitzer, M. S. (2016). Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia. PLOS ONE, 11(2), e0148616. https://doi.org/10.1371/journal.pone.0148616

Vancouver

Schultheiss M, Schnichels S, Hermann T, Hurst J, Feldkaemper M, Arango-Gonzalez B et al. Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia. PLOS ONE. 2016;11(2):e0148616. https://doi.org/10.1371/journal.pone.0148616

Bibtex

@article{ad53afe9a5b34ab59ed3c95a507b5568,
title = "Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia",
abstract = "PURPOSE: Hypothermia has been shown to be neuroprotective in the therapy of ischemic stroke in the brain. To date no studies exist on the level of the inner retina and it is unclear if hypothermia would prolong the ischemic tolerance time of retinal ganglion cells, which are decisive in many ischemic retinopathies.METHODS: Bovine eyes were enucleated and stored either at 21°C or 37°C for 100 or 340 minutes, respectively. Afterwards the globes were dissected, the retina was prepared and either the spontaneous ganglion cell responses were measured or the retina was incubated as an organotypic culture for additional 24 hours. After incubation the retina was either processed for histology (H&E and DAPI staining) or real-time PCR (Thy-1 expression) was performed.RESULTS: Hypothermia prolonged ganglion cell survival up to 340 minutes under ischemic conditions. In contrast to eyes kept at 37°C the eyes stored at 21°C still showed spontaneous ganglion cell spiking (56.8% versus 0%), a 5.8 fold higher Thy-1 mRNA expression (not significant, but a trend) and a preserved retinal structure after 340 minutes of ischemia.CONCLUSION: Hypothermia protects retinal ganglion cells against ischemia and prolongs their ischemic tolerance time.",
keywords = "Animals, Antigens, Thy-1, Cattle, Cold Temperature, Eye, Hypothermia, Induced, Ischemia, Retinal Ganglion Cells, Time Factors, Journal Article",
author = "Maximilian Schultheiss and Sven Schnichels and Thoralf Hermann and Jose Hurst and Marita Feldkaemper and Blanca Arango-Gonzalez and Marius Ueffing and Bartz-Schmidt, {Karl U} and Guenther Zeck and Spitzer, {Martin S}",
year = "2016",
doi = "10.1371/journal.pone.0148616",
language = "English",
volume = "11",
pages = "e0148616",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Hypothermia Protects and Prolongs the Tolerance Time of Retinal Ganglion Cells against Ischemia

AU - Schultheiss, Maximilian

AU - Schnichels, Sven

AU - Hermann, Thoralf

AU - Hurst, Jose

AU - Feldkaemper, Marita

AU - Arango-Gonzalez, Blanca

AU - Ueffing, Marius

AU - Bartz-Schmidt, Karl U

AU - Zeck, Guenther

AU - Spitzer, Martin S

PY - 2016

Y1 - 2016

N2 - PURPOSE: Hypothermia has been shown to be neuroprotective in the therapy of ischemic stroke in the brain. To date no studies exist on the level of the inner retina and it is unclear if hypothermia would prolong the ischemic tolerance time of retinal ganglion cells, which are decisive in many ischemic retinopathies.METHODS: Bovine eyes were enucleated and stored either at 21°C or 37°C for 100 or 340 minutes, respectively. Afterwards the globes were dissected, the retina was prepared and either the spontaneous ganglion cell responses were measured or the retina was incubated as an organotypic culture for additional 24 hours. After incubation the retina was either processed for histology (H&E and DAPI staining) or real-time PCR (Thy-1 expression) was performed.RESULTS: Hypothermia prolonged ganglion cell survival up to 340 minutes under ischemic conditions. In contrast to eyes kept at 37°C the eyes stored at 21°C still showed spontaneous ganglion cell spiking (56.8% versus 0%), a 5.8 fold higher Thy-1 mRNA expression (not significant, but a trend) and a preserved retinal structure after 340 minutes of ischemia.CONCLUSION: Hypothermia protects retinal ganglion cells against ischemia and prolongs their ischemic tolerance time.

AB - PURPOSE: Hypothermia has been shown to be neuroprotective in the therapy of ischemic stroke in the brain. To date no studies exist on the level of the inner retina and it is unclear if hypothermia would prolong the ischemic tolerance time of retinal ganglion cells, which are decisive in many ischemic retinopathies.METHODS: Bovine eyes were enucleated and stored either at 21°C or 37°C for 100 or 340 minutes, respectively. Afterwards the globes were dissected, the retina was prepared and either the spontaneous ganglion cell responses were measured or the retina was incubated as an organotypic culture for additional 24 hours. After incubation the retina was either processed for histology (H&E and DAPI staining) or real-time PCR (Thy-1 expression) was performed.RESULTS: Hypothermia prolonged ganglion cell survival up to 340 minutes under ischemic conditions. In contrast to eyes kept at 37°C the eyes stored at 21°C still showed spontaneous ganglion cell spiking (56.8% versus 0%), a 5.8 fold higher Thy-1 mRNA expression (not significant, but a trend) and a preserved retinal structure after 340 minutes of ischemia.CONCLUSION: Hypothermia protects retinal ganglion cells against ischemia and prolongs their ischemic tolerance time.

KW - Animals

KW - Antigens, Thy-1

KW - Cattle

KW - Cold Temperature

KW - Eye

KW - Hypothermia, Induced

KW - Ischemia

KW - Retinal Ganglion Cells

KW - Time Factors

KW - Journal Article

U2 - 10.1371/journal.pone.0148616

DO - 10.1371/journal.pone.0148616

M3 - SCORING: Journal article

C2 - 26848953

VL - 11

SP - e0148616

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 2

ER -