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Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa

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Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa. / Ostwald, C; Müller, P; Barten, M; Rutsatz, K; Sonnenburg, M; Milde-Langosch, K; Löning, T.

In: J ORAL PATHOL MED, Vol. 23, No. 5, 05.1994, p. 220-5.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ostwald, C, Müller, P, Barten, M, Rutsatz, K, Sonnenburg, M, Milde-Langosch, K & Löning, T 1994, 'Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa', J ORAL PATHOL MED, vol. 23, no. 5, pp. 220-5.

APA

Ostwald, C., Müller, P., Barten, M., Rutsatz, K., Sonnenburg, M., Milde-Langosch, K., & Löning, T. (1994). Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa. J ORAL PATHOL MED, 23(5), 220-5.

Vancouver

Ostwald C, Müller P, Barten M, Rutsatz K, Sonnenburg M, Milde-Langosch K et al. Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa. J ORAL PATHOL MED. 1994 May;23(5):220-5.

Bibtex

@article{4c1e3636bb794cdaa2dd01c40553f2b5,
title = "Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa",
abstract = "Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).",
keywords = "Adolescent, Adult, Aged, Aged, 80 and over, Base Sequence, Blotting, Southern, Carcinoma, Squamous Cell, Child, Child, Preschool, DNA, Viral, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Mouth Neoplasms, Oligonucleotide Probes, Papillomaviridae, Polymerase Chain Reaction",
author = "C Ostwald and P M{\"u}ller and M Barten and K Rutsatz and M Sonnenburg and K Milde-Langosch and T L{\"o}ning",
year = "1994",
month = may,
language = "English",
volume = "23",
pages = "220--5",
journal = "J ORAL PATHOL MED",
issn = "0904-2512",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa

AU - Ostwald, C

AU - Müller, P

AU - Barten, M

AU - Rutsatz, K

AU - Sonnenburg, M

AU - Milde-Langosch, K

AU - Löning, T

PY - 1994/5

Y1 - 1994/5

N2 - Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).

AB - Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).

KW - Adolescent

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Base Sequence

KW - Blotting, Southern

KW - Carcinoma, Squamous Cell

KW - Child

KW - Child, Preschool

KW - DNA, Viral

KW - Female

KW - Humans

KW - Male

KW - Middle Aged

KW - Molecular Sequence Data

KW - Mouth Neoplasms

KW - Oligonucleotide Probes

KW - Papillomaviridae

KW - Polymerase Chain Reaction

M3 - SCORING: Journal article

C2 - 8046660

VL - 23

SP - 220

EP - 225

JO - J ORAL PATHOL MED

JF - J ORAL PATHOL MED

SN - 0904-2512

IS - 5

ER -