Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa
Standard
Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa. / Ostwald, C; Müller, P; Barten, M; Rutsatz, K; Sonnenburg, M; Milde-Langosch, K; Löning, T.
in: J ORAL PATHOL MED, Jahrgang 23, Nr. 5, 05.1994, S. 220-5.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Human papillomavirus DNA in oral squamous cell carcinomas and normal mucosa
AU - Ostwald, C
AU - Müller, P
AU - Barten, M
AU - Rutsatz, K
AU - Sonnenburg, M
AU - Milde-Langosch, K
AU - Löning, T
PY - 1994/5
Y1 - 1994/5
N2 - Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).
AB - Human papillomavirus (HPV) infections in oral carcinomas and normal oral mucosa were studied by consensus primer screening and typing for HPV types 6/11, 16 and 18 DNA. After polymerase chain reaction (PCR) the DNA species of interest were identified by Southern blot hybridization with digoxigenin-labeled oligonucleotide probes. Frozen tissue and scrapings were equally suitable for HPV testing and yielded high HPV detection rates in carcinomas. By comparison, HPV analysis of paraffin-embedded material was much less efficient. HPV were demonstrated in 61.5% (16/26) of oral squamous cell carcinomas, high risk HPV 16 and 18 being the preferential types. The frequency of HPV detection in non-neoplastic mucosa of tumor patients decreased clearly with increasing distance from the tumor (range 26.9-3.8%) suggesting focal HPV infections. In contrast, normal buccal mucosa of a group of healthy volunteers contained HPV DNA only in 1% (1/97).
KW - Adolescent
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Base Sequence
KW - Blotting, Southern
KW - Carcinoma, Squamous Cell
KW - Child
KW - Child, Preschool
KW - DNA, Viral
KW - Female
KW - Humans
KW - Male
KW - Middle Aged
KW - Molecular Sequence Data
KW - Mouth Neoplasms
KW - Oligonucleotide Probes
KW - Papillomaviridae
KW - Polymerase Chain Reaction
M3 - SCORING: Journal article
C2 - 8046660
VL - 23
SP - 220
EP - 225
JO - J ORAL PATHOL MED
JF - J ORAL PATHOL MED
SN - 0904-2512
IS - 5
ER -