Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.
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Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells. / Wölpl, A; Halder, T; Kalbacher, H; Neumeyer, H; Siemoneit, K; Goldmann, S F; Eiermann, Thomas.
In: TISSUE ANTIGENS, Vol. 51, No. 3, 3, 1998, p. 258-269.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.
AU - Wölpl, A
AU - Halder, T
AU - Kalbacher, H
AU - Neumeyer, H
AU - Siemoneit, K
AU - Goldmann, S F
AU - Eiermann, Thomas
PY - 1998
Y1 - 1998
N2 - Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.
AB - Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.
KW - Humans
KW - Male
KW - Female
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Lymphocyte Activation
KW - Pregnancy
KW - Peptides
KW - Leukocytes, Mononuclear/immunology
KW - Antigen Presentation/immunology
KW - T-Lymphocytes/immunology
KW - Antibodies, Monoclonal/immunology
KW - Cell Line, Transformed
KW - Precipitin Tests
KW - Antibody Specificity/immunology
KW - HLA-DRB1 Chains
KW - Complement System Proteins/immunology
KW - Epitopes, T-Lymphocyte
KW - HLA-A2 Antigen/immunology
KW - HLA-DR Antigens/immunology
KW - HLA-DR1 Antigen/immunology
KW - Histocompatibility Antigens Class I/immunology
KW - Histocompatibility Antigens Class II/immunology
KW - T-Lymphocyte Subsets/immunology
KW - Humans
KW - Male
KW - Female
KW - Amino Acid Sequence
KW - Molecular Sequence Data
KW - Lymphocyte Activation
KW - Pregnancy
KW - Peptides
KW - Leukocytes, Mononuclear/immunology
KW - Antigen Presentation/immunology
KW - T-Lymphocytes/immunology
KW - Antibodies, Monoclonal/immunology
KW - Cell Line, Transformed
KW - Precipitin Tests
KW - Antibody Specificity/immunology
KW - HLA-DRB1 Chains
KW - Complement System Proteins/immunology
KW - Epitopes, T-Lymphocyte
KW - HLA-A2 Antigen/immunology
KW - HLA-DR Antigens/immunology
KW - HLA-DR1 Antigen/immunology
KW - Histocompatibility Antigens Class I/immunology
KW - Histocompatibility Antigens Class II/immunology
KW - T-Lymphocyte Subsets/immunology
M3 - SCORING: Journal article
VL - 51
SP - 258
EP - 269
JO - TISSUE ANTIGENS
JF - TISSUE ANTIGENS
SN - 0001-2815
IS - 3
M1 - 3
ER -