Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.

A Wölpl; T Halder; H Kalbacher; H Neumeyer; K Siemoneit; S F Goldmann; Thomas Eiermann

Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.

Standard

Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells. / Wölpl, A; Halder, T; Kalbacher, H; Neumeyer, H; Siemoneit, K; Goldmann, S F; Eiermann, Thomas.

in: TISSUE ANTIGENS, Jahrgang 51, Nr. 3, 3, 1998, S. 258-269.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Wölpl, A, Halder, T, Kalbacher, H, Neumeyer, H, Siemoneit, K, Goldmann, SF & Eiermann, T 1998, 'Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.', TISSUE ANTIGENS, Jg. 51, Nr. 3, 3, S. 258-269. <http://www.ncbi.nlm.nih.gov/pubmed/9550326?dopt=Citation>

APA

Wölpl, A., Halder, T., Kalbacher, H., Neumeyer, H., Siemoneit, K., Goldmann, S. F., & Eiermann, T. (1998). Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells. TISSUE ANTIGENS, 51(3), 258-269. [3]. http://www.ncbi.nlm.nih.gov/pubmed/9550326?dopt=Citation

Vancouver

Wölpl A, Halder T, Kalbacher H, Neumeyer H, Siemoneit K, Goldmann SF et al. Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells. TISSUE ANTIGENS. 1998;51(3):258-269. 3.

Bibtex

@article{b06a77dbe73746ae979ade2dda14ff61,

title = "Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.",

abstract = "Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.",

keywords = "Humans, Male, Female, Amino Acid Sequence, Molecular Sequence Data, Lymphocyte Activation, Pregnancy, Peptides, Leukocytes, Mononuclear/immunology, Antigen Presentation/*immunology, T-Lymphocytes/*immunology, Antibodies, Monoclonal/*immunology, Cell Line, Transformed, Precipitin Tests, Antibody Specificity/*immunology, HLA-DRB1 Chains, Complement System Proteins/immunology, Epitopes, T-Lymphocyte, HLA-A2 Antigen/immunology, HLA-DR Antigens/immunology, HLA-DR1 Antigen/*immunology, Histocompatibility Antigens Class I/*immunology, Histocompatibility Antigens Class II/immunology, T-Lymphocyte Subsets/immunology, Humans, Male, Female, Amino Acid Sequence, Molecular Sequence Data, Lymphocyte Activation, Pregnancy, Peptides, Leukocytes, Mononuclear/immunology, Antigen Presentation/*immunology, T-Lymphocytes/*immunology, Antibodies, Monoclonal/*immunology, Cell Line, Transformed, Precipitin Tests, Antibody Specificity/*immunology, HLA-DRB1 Chains, Complement System Proteins/immunology, Epitopes, T-Lymphocyte, HLA-A2 Antigen/immunology, HLA-DR Antigens/immunology, HLA-DR1 Antigen/*immunology, Histocompatibility Antigens Class I/*immunology, Histocompatibility Antigens Class II/immunology, T-Lymphocyte Subsets/immunology",

author = "A W{\"o}lpl and T Halder and H Kalbacher and H Neumeyer and K Siemoneit and Goldmann, {S F} and Thomas Eiermann",

year = "1998",

language = "English",

volume = "51",

pages = "258--269",

journal = "TISSUE ANTIGENS",

issn = "0001-2815",

publisher = "Wiley-Blackwell",

number = "3",

}

RIS

TY - JOUR

T1 - Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells.

AU - Wölpl, A

AU - Halder, T

AU - Kalbacher, H

AU - Neumeyer, H

AU - Siemoneit, K

AU - Goldmann, S F

AU - Eiermann, Thomas

PY - 1998

Y1 - 1998

N2 - Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.

AB - Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.

KW - Humans

KW - Male

KW - Female

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - Lymphocyte Activation

KW - Pregnancy

KW - Peptides

KW - Leukocytes, Mononuclear/immunology

KW - Antigen Presentation/immunology

KW - T-Lymphocytes/immunology

KW - Antibodies, Monoclonal/immunology

KW - Cell Line, Transformed

KW - Precipitin Tests

KW - Antibody Specificity/immunology

KW - HLA-DRB1 Chains

KW - Complement System Proteins/immunology

KW - Epitopes, T-Lymphocyte

KW - HLA-A2 Antigen/immunology

KW - HLA-DR Antigens/immunology

KW - HLA-DR1 Antigen/immunology

KW - Histocompatibility Antigens Class I/immunology

KW - Histocompatibility Antigens Class II/immunology

KW - T-Lymphocyte Subsets/immunology