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Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection

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Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection. / Ravens, Sarina; Schultze-Florey, Christian; Raha, Solaiman; Sandrock, Inga; Drenker, Melanie; Oberdörfer, Linda; Reinhardt, Annika; Ravens, Inga; Beck, Maleen; Geffers, Robert; von Kaisenberg, Constantin; Heuser, Michael; Thol, Felicitas; Ganser, Arnold; Förster, Reinhold; Koenecke, Christian; Prinz, Immo.

In: NAT IMMUNOL, Vol. 18, No. 4, 04.2017, p. 393-401.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ravens, S, Schultze-Florey, C, Raha, S, Sandrock, I, Drenker, M, Oberdörfer, L, Reinhardt, A, Ravens, I, Beck, M, Geffers, R, von Kaisenberg, C, Heuser, M, Thol, F, Ganser, A, Förster, R, Koenecke, C & Prinz, I 2017, 'Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection', NAT IMMUNOL, vol. 18, no. 4, pp. 393-401. https://doi.org/10.1038/ni.3686

APA

Ravens, S., Schultze-Florey, C., Raha, S., Sandrock, I., Drenker, M., Oberdörfer, L., Reinhardt, A., Ravens, I., Beck, M., Geffers, R., von Kaisenberg, C., Heuser, M., Thol, F., Ganser, A., Förster, R., Koenecke, C., & Prinz, I. (2017). Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection. NAT IMMUNOL, 18(4), 393-401. https://doi.org/10.1038/ni.3686

Vancouver

Ravens S, Schultze-Florey C, Raha S, Sandrock I, Drenker M, Oberdörfer L et al. Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection. NAT IMMUNOL. 2017 Apr;18(4):393-401. https://doi.org/10.1038/ni.3686

Bibtex

@article{15b55b9e60a241baaff14e3ffc63fd4d,
title = "Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection",
abstract = "To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.",
keywords = "Clonal Evolution/genetics, Cytomegalovirus Infections/genetics, Gene Rearrangement, T-Lymphocyte, Graft Survival, Hematopoietic Stem Cell Transplantation, Humans, Lymphocyte Activation/genetics, Receptors, Antigen, T-Cell, gamma-delta/genetics, T-Lymphocyte Subsets/immunology, Transplantation, Homologous",
author = "Sarina Ravens and Christian Schultze-Florey and Solaiman Raha and Inga Sandrock and Melanie Drenker and Linda Oberd{\"o}rfer and Annika Reinhardt and Inga Ravens and Maleen Beck and Robert Geffers and {von Kaisenberg}, Constantin and Michael Heuser and Felicitas Thol and Arnold Ganser and Reinhold F{\"o}rster and Christian Koenecke and Immo Prinz",
year = "2017",
month = apr,
doi = "10.1038/ni.3686",
language = "English",
volume = "18",
pages = "393--401",
journal = "NAT IMMUNOL",
issn = "1529-2908",
publisher = "NATURE PUBLISHING GROUP",
number = "4",

}

RIS

TY - JOUR

T1 - Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection

AU - Ravens, Sarina

AU - Schultze-Florey, Christian

AU - Raha, Solaiman

AU - Sandrock, Inga

AU - Drenker, Melanie

AU - Oberdörfer, Linda

AU - Reinhardt, Annika

AU - Ravens, Inga

AU - Beck, Maleen

AU - Geffers, Robert

AU - von Kaisenberg, Constantin

AU - Heuser, Michael

AU - Thol, Felicitas

AU - Ganser, Arnold

AU - Förster, Reinhold

AU - Koenecke, Christian

AU - Prinz, Immo

PY - 2017/4

Y1 - 2017/4

N2 - To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.

AB - To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.

KW - Clonal Evolution/genetics

KW - Cytomegalovirus Infections/genetics

KW - Gene Rearrangement, T-Lymphocyte

KW - Graft Survival

KW - Hematopoietic Stem Cell Transplantation

KW - Humans

KW - Lymphocyte Activation/genetics

KW - Receptors, Antigen, T-Cell, gamma-delta/genetics

KW - T-Lymphocyte Subsets/immunology

KW - Transplantation, Homologous

U2 - 10.1038/ni.3686

DO - 10.1038/ni.3686

M3 - SCORING: Journal article

C2 - 28218745

VL - 18

SP - 393

EP - 401

JO - NAT IMMUNOL

JF - NAT IMMUNOL

SN - 1529-2908

IS - 4

ER -