Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection
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Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection. / Ravens, Sarina; Schultze-Florey, Christian; Raha, Solaiman; Sandrock, Inga; Drenker, Melanie; Oberdörfer, Linda; Reinhardt, Annika; Ravens, Inga; Beck, Maleen; Geffers, Robert; von Kaisenberg, Constantin; Heuser, Michael; Thol, Felicitas; Ganser, Arnold; Förster, Reinhold; Koenecke, Christian; Prinz, Immo.
in: NAT IMMUNOL, Jahrgang 18, Nr. 4, 04.2017, S. 393-401.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Human γδ T cells are quickly reconstituted after stem-cell transplantation and show adaptive clonal expansion in response to viral infection
AU - Ravens, Sarina
AU - Schultze-Florey, Christian
AU - Raha, Solaiman
AU - Sandrock, Inga
AU - Drenker, Melanie
AU - Oberdörfer, Linda
AU - Reinhardt, Annika
AU - Ravens, Inga
AU - Beck, Maleen
AU - Geffers, Robert
AU - von Kaisenberg, Constantin
AU - Heuser, Michael
AU - Thol, Felicitas
AU - Ganser, Arnold
AU - Förster, Reinhold
AU - Koenecke, Christian
AU - Prinz, Immo
PY - 2017/4
Y1 - 2017/4
N2 - To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.
AB - To investigate how the human γδ T cell pool is shaped during ontogeny and how it is regenerated after transplantation of hematopoietic stem cells (HSCs), we applied an RNA-based next-generation sequencing approach to monitor the dynamics of the repertoires of γδ T cell antigen receptors (TCRs) before and after transplantation in a prospective cohort study. We found that repertoires of rearranged genes encoding γδ TCRs (TRG and TRD) in the peripheral blood of healthy adults were stable over time. Although a large fraction of human TRG repertoires consisted of public sequences, the TRD repertoires were private. In patients undergoing HSC transplantation, γδ T cells were quickly reconstituted; however, they had profoundly altered TCR repertoires. Notably, the clonal proliferation of individual virus-reactive γδ TCR sequences in patients with reactivation of cytomegalovirus revealed strong evidence for adaptive anti-viral γδ T cell immune responses.
KW - Clonal Evolution/genetics
KW - Cytomegalovirus Infections/genetics
KW - Gene Rearrangement, T-Lymphocyte
KW - Graft Survival
KW - Hematopoietic Stem Cell Transplantation
KW - Humans
KW - Lymphocyte Activation/genetics
KW - Receptors, Antigen, T-Cell, gamma-delta/genetics
KW - T-Lymphocyte Subsets/immunology
KW - Transplantation, Homologous
U2 - 10.1038/ni.3686
DO - 10.1038/ni.3686
M3 - SCORING: Journal article
C2 - 28218745
VL - 18
SP - 393
EP - 401
JO - NAT IMMUNOL
JF - NAT IMMUNOL
SN - 1529-2908
IS - 4
ER -