Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells
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Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells. / Follain, Gautier; Osmani, Naël; Azevedo, Ana Sofia; Allio, Guillaume; Mercier, Luc; Karreman, Matthia A; Solecki, Gergely; Garcia Leòn, Marìa Jesùs; Lefebvre, Olivier; Fekonja, Nina; Hille, Claudia; Chabannes, Vincent; Dollé, Guillaume; Metivet, Thibaut; Hovsepian, François Der; Prudhomme, Christophe; Pichot, Angélique; Paul, Nicodème; Carapito, Raphaël; Bahram, Siamak; Ruthensteiner, Bernhard; Kemmling, André; Siemonsen, Susanne; Schneider, Tanja; Fiehler, Jens; Glatzel, Markus; Winkler, Frank; Schwab, Yannick; Pantel, Klaus; Harlepp, Sébastien; Goetz, Jacky G.
In: DEV CELL, Vol. 45, No. 1, 09.04.2018, p. 33-52.e12.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Hemodynamic Forces Tune the Arrest, Adhesion, and Extravasation of Circulating Tumor Cells
AU - Follain, Gautier
AU - Osmani, Naël
AU - Azevedo, Ana Sofia
AU - Allio, Guillaume
AU - Mercier, Luc
AU - Karreman, Matthia A
AU - Solecki, Gergely
AU - Garcia Leòn, Marìa Jesùs
AU - Lefebvre, Olivier
AU - Fekonja, Nina
AU - Hille, Claudia
AU - Chabannes, Vincent
AU - Dollé, Guillaume
AU - Metivet, Thibaut
AU - Hovsepian, François Der
AU - Prudhomme, Christophe
AU - Pichot, Angélique
AU - Paul, Nicodème
AU - Carapito, Raphaël
AU - Bahram, Siamak
AU - Ruthensteiner, Bernhard
AU - Kemmling, André
AU - Siemonsen, Susanne
AU - Schneider, Tanja
AU - Fiehler, Jens
AU - Glatzel, Markus
AU - Winkler, Frank
AU - Schwab, Yannick
AU - Pantel, Klaus
AU - Harlepp, Sébastien
AU - Goetz, Jacky G
N1 - Copyright © 2018 Elsevier Inc. All rights reserved.
PY - 2018/4/9
Y1 - 2018/4/9
N2 - Metastatic seeding is driven by cell-intrinsic and environmental cues, yet the contribution of biomechanics is poorly known. We aim to elucidate the impact of blood flow on the arrest and the extravasation of circulating tumor cells (CTCs) in vivo. Using the zebrafish embryo, we show that arrest of CTCs occurs in vessels with favorable flow profiles where flow forces control the adhesion efficacy of CTCs to the endothelium. We biophysically identified the threshold values of flow and adhesion forces allowing successful arrest of CTCs. In addition, flow forces fine-tune tumor cell extravasation by impairing the remodeling properties of the endothelium. Importantly, we also observe endothelial remodeling at arrest sites of CTCs in mouse brain capillaries. Finally, we observed that human supratentorial brain metastases preferably develop in areas with low perfusion. These results demonstrate that hemodynamic profiles at metastatic sites regulate key steps of extravasation preceding metastatic outgrowth.
AB - Metastatic seeding is driven by cell-intrinsic and environmental cues, yet the contribution of biomechanics is poorly known. We aim to elucidate the impact of blood flow on the arrest and the extravasation of circulating tumor cells (CTCs) in vivo. Using the zebrafish embryo, we show that arrest of CTCs occurs in vessels with favorable flow profiles where flow forces control the adhesion efficacy of CTCs to the endothelium. We biophysically identified the threshold values of flow and adhesion forces allowing successful arrest of CTCs. In addition, flow forces fine-tune tumor cell extravasation by impairing the remodeling properties of the endothelium. Importantly, we also observe endothelial remodeling at arrest sites of CTCs in mouse brain capillaries. Finally, we observed that human supratentorial brain metastases preferably develop in areas with low perfusion. These results demonstrate that hemodynamic profiles at metastatic sites regulate key steps of extravasation preceding metastatic outgrowth.
KW - Journal Article
U2 - 10.1016/j.devcel.2018.02.015
DO - 10.1016/j.devcel.2018.02.015
M3 - SCORING: Journal article
C2 - 29634935
VL - 45
SP - 33-52.e12
JO - DEV CELL
JF - DEV CELL
SN - 1534-5807
IS - 1
ER -