Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
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Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells. / Nowacki, Tobias M; Kuerten, Stefanie; Zhang, Wenji; Shive, Carey L; Kreher, Christian; Boehm, Bernhard O; Lehmann, Paul V; Tary-Lehmann, Magdalena.
In: CELL IMMUNOL, Vol. 247, No. 1, 1, 2007, p. 36-48.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
AU - Nowacki, Tobias M
AU - Kuerten, Stefanie
AU - Zhang, Wenji
AU - Shive, Carey L
AU - Kreher, Christian
AU - Boehm, Bernhard O
AU - Lehmann, Paul V
AU - Tary-Lehmann, Magdalena
PY - 2007
Y1 - 2007
N2 - For immune diagnostic purposes it would be critical to be able to distinguish between ongoing immune processes, such as active infections, and long-term immune memory, for example imprinted by infections that have been cleared a long time ago or by vaccinations. We tested the hypothesis that the secretion of granzyme B, as detected in ex vivo ELISPOT assays, permits this distinction. We studied EBV-, flu- and CMV-specific CD8(+) cells in healthy individuals, Vaccinia virus-reactive CD8(+) cells in the course of vaccination, and HIV-specific CD8(+) cells in HIV-infected individuals. Antigen-specific ex vivo GzB production was detected only transiently after Vaccinia immunization, and in HIV-infected individuals. Our data suggest that ex vivo ELISPOT measurements of granzyme B permit the identification of actively ongoing CD8(+) cell responses-a notion that is pertinent to the immune diagnostic of infections, transplantation, allergies, autoimmune diseases, tumors and vaccine development.
AB - For immune diagnostic purposes it would be critical to be able to distinguish between ongoing immune processes, such as active infections, and long-term immune memory, for example imprinted by infections that have been cleared a long time ago or by vaccinations. We tested the hypothesis that the secretion of granzyme B, as detected in ex vivo ELISPOT assays, permits this distinction. We studied EBV-, flu- and CMV-specific CD8(+) cells in healthy individuals, Vaccinia virus-reactive CD8(+) cells in the course of vaccination, and HIV-specific CD8(+) cells in HIV-infected individuals. Antigen-specific ex vivo GzB production was detected only transiently after Vaccinia immunization, and in HIV-infected individuals. Our data suggest that ex vivo ELISPOT measurements of granzyme B permit the identification of actively ongoing CD8(+) cell responses-a notion that is pertinent to the immune diagnostic of infections, transplantation, allergies, autoimmune diseases, tumors and vaccine development.
M3 - SCORING: Zeitschriftenaufsatz
VL - 247
SP - 36
EP - 48
JO - CELL IMMUNOL
JF - CELL IMMUNOL
SN - 0008-8749
IS - 1
M1 - 1
ER -