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Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori

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Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori. / Lehours, Philippe; Dupouy, Sandrine; Chaineux, Julien; Ruskoné-Fourmestraux, Agnès; Delchier, Jean-Charles; Morgner, Andrea; Mégraud, Francis; Ménard, Armelle.

In: RES MICROBIOL, Vol. 158, No. 3, 04.2007, p. 265-71.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lehours, P, Dupouy, S, Chaineux, J, Ruskoné-Fourmestraux, A, Delchier, J-C, Morgner, A, Mégraud, F & Ménard, A 2007, 'Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori', RES MICROBIOL, vol. 158, no. 3, pp. 265-71. https://doi.org/10.1016/j.resmic.2006.12.002

APA

Lehours, P., Dupouy, S., Chaineux, J., Ruskoné-Fourmestraux, A., Delchier, J-C., Morgner, A., Mégraud, F., & Ménard, A. (2007). Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori. RES MICROBIOL, 158(3), 265-71. https://doi.org/10.1016/j.resmic.2006.12.002

Vancouver

Lehours P, Dupouy S, Chaineux J, Ruskoné-Fourmestraux A, Delchier J-C, Morgner A et al. Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori. RES MICROBIOL. 2007 Apr;158(3):265-71. https://doi.org/10.1016/j.resmic.2006.12.002

Bibtex

@article{c020df69003f4194ba7dfb4fe62ccdb0,
title = "Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori",
abstract = "Helicobacter pylori is unique because of the unusually high number and diversity of its restriction modification (R-M) systems. HpyC1I R-M was recently characterized and contains an endonuclease which is an isoschizomer of the endonuclease BccI. This R-M is involved in adherence to gastric epithelial cells, a crucial step in bacterial pathogenesis. This observation illustrates the fact that R-M systems have other putative biological functions in addition to protecting the bacterial genome from external DNA. The genomic diversity of HpyC1I R-M was evaluated more precisely on a large collection of H. pylori strains by PCR, susceptibility to BccI digestion and sequencing. The results obtained support the mechanism of gain and loss of this R-M system in the H. pylori genome, and suggest that it is an ancestral system which gradually disappears during H. pylori evolution, following successive steps: (1) inactivation of the endonuclease gene, followed or accompanied by: (2) inactivation of the methyltransferase genes, and then: (3) definitive loss, leaving only short endonuclease remnant sequences.",
keywords = "Amino Acid Sequence, Cloning, Molecular, DNA Restriction-Modification Enzymes, DNA, Bacterial, Deoxyribonucleases, Type II Site-Specific, Gene Order, Genetic Variation, Genome, Bacterial, Helicobacter pylori, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Journal Article, Research Support, Non-U.S. Gov't",
author = "Philippe Lehours and Sandrine Dupouy and Julien Chaineux and Agn{\`e}s Ruskon{\'e}-Fourmestraux and Jean-Charles Delchier and Andrea Morgner and Francis M{\'e}graud and Armelle M{\'e}nard",
year = "2007",
month = apr,
doi = "10.1016/j.resmic.2006.12.002",
language = "English",
volume = "158",
pages = "265--71",
journal = "RES MICROBIOL",
issn = "0923-2508",
publisher = "Elsevier Masson SAS",
number = "3",

}

RIS

TY - JOUR

T1 - Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori

AU - Lehours, Philippe

AU - Dupouy, Sandrine

AU - Chaineux, Julien

AU - Ruskoné-Fourmestraux, Agnès

AU - Delchier, Jean-Charles

AU - Morgner, Andrea

AU - Mégraud, Francis

AU - Ménard, Armelle

PY - 2007/4

Y1 - 2007/4

N2 - Helicobacter pylori is unique because of the unusually high number and diversity of its restriction modification (R-M) systems. HpyC1I R-M was recently characterized and contains an endonuclease which is an isoschizomer of the endonuclease BccI. This R-M is involved in adherence to gastric epithelial cells, a crucial step in bacterial pathogenesis. This observation illustrates the fact that R-M systems have other putative biological functions in addition to protecting the bacterial genome from external DNA. The genomic diversity of HpyC1I R-M was evaluated more precisely on a large collection of H. pylori strains by PCR, susceptibility to BccI digestion and sequencing. The results obtained support the mechanism of gain and loss of this R-M system in the H. pylori genome, and suggest that it is an ancestral system which gradually disappears during H. pylori evolution, following successive steps: (1) inactivation of the endonuclease gene, followed or accompanied by: (2) inactivation of the methyltransferase genes, and then: (3) definitive loss, leaving only short endonuclease remnant sequences.

AB - Helicobacter pylori is unique because of the unusually high number and diversity of its restriction modification (R-M) systems. HpyC1I R-M was recently characterized and contains an endonuclease which is an isoschizomer of the endonuclease BccI. This R-M is involved in adherence to gastric epithelial cells, a crucial step in bacterial pathogenesis. This observation illustrates the fact that R-M systems have other putative biological functions in addition to protecting the bacterial genome from external DNA. The genomic diversity of HpyC1I R-M was evaluated more precisely on a large collection of H. pylori strains by PCR, susceptibility to BccI digestion and sequencing. The results obtained support the mechanism of gain and loss of this R-M system in the H. pylori genome, and suggest that it is an ancestral system which gradually disappears during H. pylori evolution, following successive steps: (1) inactivation of the endonuclease gene, followed or accompanied by: (2) inactivation of the methyltransferase genes, and then: (3) definitive loss, leaving only short endonuclease remnant sequences.

KW - Amino Acid Sequence

KW - Cloning, Molecular

KW - DNA Restriction-Modification Enzymes

KW - DNA, Bacterial

KW - Deoxyribonucleases, Type II Site-Specific

KW - Gene Order

KW - Genetic Variation

KW - Genome, Bacterial

KW - Helicobacter pylori

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.resmic.2006.12.002

DO - 10.1016/j.resmic.2006.12.002

M3 - SCORING: Journal article

C2 - 17346936

VL - 158

SP - 265

EP - 271

JO - RES MICROBIOL

JF - RES MICROBIOL

SN - 0923-2508

IS - 3

ER -