Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori
Standard
Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori. / Lehours, Philippe; Dupouy, Sandrine; Chaineux, Julien; Ruskoné-Fourmestraux, Agnès; Delchier, Jean-Charles; Morgner, Andrea; Mégraud, Francis; Ménard, Armelle.
in: RES MICROBIOL, Jahrgang 158, Nr. 3, 04.2007, S. 265-71.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Genetic diversity of the HpyC1I restriction modification system in Helicobacter pylori
AU - Lehours, Philippe
AU - Dupouy, Sandrine
AU - Chaineux, Julien
AU - Ruskoné-Fourmestraux, Agnès
AU - Delchier, Jean-Charles
AU - Morgner, Andrea
AU - Mégraud, Francis
AU - Ménard, Armelle
PY - 2007/4
Y1 - 2007/4
N2 - Helicobacter pylori is unique because of the unusually high number and diversity of its restriction modification (R-M) systems. HpyC1I R-M was recently characterized and contains an endonuclease which is an isoschizomer of the endonuclease BccI. This R-M is involved in adherence to gastric epithelial cells, a crucial step in bacterial pathogenesis. This observation illustrates the fact that R-M systems have other putative biological functions in addition to protecting the bacterial genome from external DNA. The genomic diversity of HpyC1I R-M was evaluated more precisely on a large collection of H. pylori strains by PCR, susceptibility to BccI digestion and sequencing. The results obtained support the mechanism of gain and loss of this R-M system in the H. pylori genome, and suggest that it is an ancestral system which gradually disappears during H. pylori evolution, following successive steps: (1) inactivation of the endonuclease gene, followed or accompanied by: (2) inactivation of the methyltransferase genes, and then: (3) definitive loss, leaving only short endonuclease remnant sequences.
AB - Helicobacter pylori is unique because of the unusually high number and diversity of its restriction modification (R-M) systems. HpyC1I R-M was recently characterized and contains an endonuclease which is an isoschizomer of the endonuclease BccI. This R-M is involved in adherence to gastric epithelial cells, a crucial step in bacterial pathogenesis. This observation illustrates the fact that R-M systems have other putative biological functions in addition to protecting the bacterial genome from external DNA. The genomic diversity of HpyC1I R-M was evaluated more precisely on a large collection of H. pylori strains by PCR, susceptibility to BccI digestion and sequencing. The results obtained support the mechanism of gain and loss of this R-M system in the H. pylori genome, and suggest that it is an ancestral system which gradually disappears during H. pylori evolution, following successive steps: (1) inactivation of the endonuclease gene, followed or accompanied by: (2) inactivation of the methyltransferase genes, and then: (3) definitive loss, leaving only short endonuclease remnant sequences.
KW - Amino Acid Sequence
KW - Cloning, Molecular
KW - DNA Restriction-Modification Enzymes
KW - DNA, Bacterial
KW - Deoxyribonucleases, Type II Site-Specific
KW - Gene Order
KW - Genetic Variation
KW - Genome, Bacterial
KW - Helicobacter pylori
KW - Molecular Sequence Data
KW - Polymerase Chain Reaction
KW - Sequence Analysis, DNA
KW - Sequence Homology, Amino Acid
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.resmic.2006.12.002
DO - 10.1016/j.resmic.2006.12.002
M3 - SCORING: Journal article
C2 - 17346936
VL - 158
SP - 265
EP - 271
JO - RES MICROBIOL
JF - RES MICROBIOL
SN - 0923-2508
IS - 3
ER -