Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP

Roberta Kurelić; Viacheslav O Nikolaev

doi:10.1007/978-1-0716-2245-2_15

Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP

Standard

Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP. / Kurelić, Roberta ; Nikolaev, Viacheslav O.

cAMP Signaling: Methods and Protocols. ed. / Manuela Zaccolo. 2. ed. New York, NY : Humana Press, 2022. p. 241-254 (Methods in Molecular Biology; Vol. 2483).

Research output: SCORING: Contribution to book/anthology › SCORING: Contribution to collected editions/anthologies › Research

Harvard

Kurelić, R & Nikolaev, VO 2022, Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP. in M Zaccolo (ed.), cAMP Signaling: Methods and Protocols. 2 edn, Methods in Molecular Biology, vol. 2483, Humana Press, New York, NY, pp. 241-254. https://doi.org/10.1007/978-1-0716-2245-2_15

APA

Kurelić, R., & Nikolaev, V. O. (2022). Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP. In M. Zaccolo (Ed.), cAMP Signaling: Methods and Protocols (2 ed., pp. 241-254). (Methods in Molecular Biology; Vol. 2483). Humana Press. https://doi.org/10.1007/978-1-0716-2245-2_15

Vancouver

Kurelić R , Nikolaev VO. Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP. In Zaccolo M, editor, cAMP Signaling: Methods and Protocols. 2 ed. New York, NY: Humana Press. 2022. p. 241-254. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-0716-2245-2_15

Bibtex

@inbook{5a2161c94df6459992bdb10e825ba5ab,

title = "Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP",

abstract = "Transgenic mice play a significant role in modern biomedical research. In addition to mechanistic studies of a specific gene and protein function, transgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice ubiquitously or constitutively expressing cytosolic and targeted F{\"o}rster resonance energy transfer (FRET)-based biosensors for the second messengers 3',5'-cyclic adenosine and guanosine monophosphates. These tools and techniques hold great potential for the analysis of second messenger dynamics in physiologically relevant systems.",

keywords = "Animals, Biosensing Techniques/methods, Cyclic GMP/metabolism, Fluorescence Resonance Energy Transfer/methods, Mice, Mice, Transgenic, Second Messenger Systems",

author = "Roberta Kureli{\'c} and Nikolaev, {Viacheslav O}",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2245-2_15",

language = "English",

isbn = "978-1-0716-2244-5",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "241--254",

editor = "Manuela Zaccolo",

booktitle = "cAMP Signaling",

address = "United States",

edition = "2",

}

RIS

TY - CHAP

T1 - Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP

AU - Kurelić, Roberta

AU - Nikolaev, Viacheslav O

PY - 2022

Y1 - 2022

N2 - Transgenic mice play a significant role in modern biomedical research. In addition to mechanistic studies of a specific gene and protein function, transgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice ubiquitously or constitutively expressing cytosolic and targeted Förster resonance energy transfer (FRET)-based biosensors for the second messengers 3',5'-cyclic adenosine and guanosine monophosphates. These tools and techniques hold great potential for the analysis of second messenger dynamics in physiologically relevant systems.

AB - Transgenic mice play a significant role in modern biomedical research. In addition to mechanistic studies of a specific gene and protein function, transgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice ubiquitously or constitutively expressing cytosolic and targeted Förster resonance energy transfer (FRET)-based biosensors for the second messengers 3',5'-cyclic adenosine and guanosine monophosphates. These tools and techniques hold great potential for the analysis of second messenger dynamics in physiologically relevant systems.

KW - Animals

KW - Biosensing Techniques/methods

KW - Cyclic GMP/metabolism

KW - Fluorescence Resonance Energy Transfer/methods

KW - Mice

KW - Mice, Transgenic

KW - Second Messenger Systems

U2 - 10.1007/978-1-0716-2245-2_15

DO - 10.1007/978-1-0716-2245-2_15

M3 - SCORING: Contribution to collected editions/anthologies

C2 - 35286680

SN - 978-1-0716-2244-5

SN - 978-1-0716-2247-6

T3 - Methods in Molecular Biology

SP - 241

EP - 254

BT - cAMP Signaling

A2 - Zaccolo, Manuela

PB - Humana Press

CY - New York, NY

ER -