Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP
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Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP. / Kurelić, Roberta; Nikolaev, Viacheslav O.
cAMP Signaling: Methods and Protocols. Hrsg. / Manuela Zaccolo. 2. Aufl. New York, NY : Humana Press, 2022. S. 241-254 (Methods in Molecular Biology; Band 2483).Publikationen: SCORING: Beitrag in Buch/Sammelwerk › SCORING: Beitrag in Sammelwerk › Forschung
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TY - CHAP
T1 - Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP
AU - Kurelić, Roberta
AU - Nikolaev, Viacheslav O
N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Transgenic mice play a significant role in modern biomedical research. In addition to mechanistic studies of a specific gene and protein function, transgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice ubiquitously or constitutively expressing cytosolic and targeted Förster resonance energy transfer (FRET)-based biosensors for the second messengers 3',5'-cyclic adenosine and guanosine monophosphates. These tools and techniques hold great potential for the analysis of second messenger dynamics in physiologically relevant systems.
AB - Transgenic mice play a significant role in modern biomedical research. In addition to mechanistic studies of a specific gene and protein function, transgenic mice are used as an exciting tool for in vivo or in situ analysis of fluorescent biosensors, which are capable of directly reporting second messenger levels and biochemical processes in real time and living cells. In this chapter, we present a detailed protocol for the generation of plasmid vectors and transgenic mice ubiquitously or constitutively expressing cytosolic and targeted Förster resonance energy transfer (FRET)-based biosensors for the second messengers 3',5'-cyclic adenosine and guanosine monophosphates. These tools and techniques hold great potential for the analysis of second messenger dynamics in physiologically relevant systems.
KW - Animals
KW - Biosensing Techniques/methods
KW - Cyclic GMP/metabolism
KW - Fluorescence Resonance Energy Transfer/methods
KW - Mice
KW - Mice, Transgenic
KW - Second Messenger Systems
U2 - 10.1007/978-1-0716-2245-2_15
DO - 10.1007/978-1-0716-2245-2_15
M3 - SCORING: Contribution to collected editions/anthologies
C2 - 35286680
SN - 978-1-0716-2244-5
SN - 978-1-0716-2247-6
T3 - Methods in Molecular Biology
SP - 241
EP - 254
BT - cAMP Signaling
A2 - Zaccolo, Manuela
PB - Humana Press
CY - New York, NY
ER -