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Generation of angiotensin II from human plasma by tissue kallikrein

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Generation of angiotensin II from human plasma by tissue kallikrein. / Krivoy, N; Schlüter, H; Karas, M; Zidek, W.

In: CLIN SCI, Vol. 83, No. 4, 10.1992, p. 477-82.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Krivoy, N, Schlüter, H, Karas, M & Zidek, W 1992, 'Generation of angiotensin II from human plasma by tissue kallikrein', CLIN SCI, vol. 83, no. 4, pp. 477-82.

APA

Krivoy, N., Schlüter, H., Karas, M., & Zidek, W. (1992). Generation of angiotensin II from human plasma by tissue kallikrein. CLIN SCI, 83(4), 477-82.

Vancouver

Krivoy N, Schlüter H, Karas M, Zidek W. Generation of angiotensin II from human plasma by tissue kallikrein. CLIN SCI. 1992 Oct;83(4):477-82.

Bibtex

@article{fa4f735e29324598a7d7259e2a7ab3a1,
title = "Generation of angiotensin II from human plasma by tissue kallikrein",
abstract = "1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.",
keywords = "Angiotensin II, Animals, Aprotinin, Blood Pressure, Dose-Response Relationship, Drug, Humans, Kallikreins, Kidney, Mass Spectrometry, Norepinephrine, Rats, Rats, Inbred WKY, Saralasin, Journal Article",
author = "N Krivoy and H Schl{\"u}ter and M Karas and W Zidek",
year = "1992",
month = oct,
language = "English",
volume = "83",
pages = "477--82",
journal = "CLIN SCI",
issn = "0143-5221",
publisher = "PORTLAND PRESS LTD",
number = "4",

}

RIS

TY - JOUR

T1 - Generation of angiotensin II from human plasma by tissue kallikrein

AU - Krivoy, N

AU - Schlüter, H

AU - Karas, M

AU - Zidek, W

PY - 1992/10

Y1 - 1992/10

N2 - 1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.

AB - 1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.

KW - Angiotensin II

KW - Animals

KW - Aprotinin

KW - Blood Pressure

KW - Dose-Response Relationship, Drug

KW - Humans

KW - Kallikreins

KW - Kidney

KW - Mass Spectrometry

KW - Norepinephrine

KW - Rats

KW - Rats, Inbred WKY

KW - Saralasin

KW - Journal Article

M3 - SCORING: Journal article

C2 - 1385031

VL - 83

SP - 477

EP - 482

JO - CLIN SCI

JF - CLIN SCI

SN - 0143-5221

IS - 4

ER -