Generation of angiotensin II from human plasma by tissue kallikrein
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Generation of angiotensin II from human plasma by tissue kallikrein. / Krivoy, N; Schlüter, H; Karas, M; Zidek, W.
in: CLIN SCI, Jahrgang 83, Nr. 4, 10.1992, S. 477-82.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Generation of angiotensin II from human plasma by tissue kallikrein
AU - Krivoy, N
AU - Schlüter, H
AU - Karas, M
AU - Zidek, W
PY - 1992/10
Y1 - 1992/10
N2 - 1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.
AB - 1. Human plasma was incubated with tissue kallikrein from porcine pancreas, dialysed to obtain a fraction with a molecular mass < 10 kDa and further purified by reverse-phase chromatography. 2. Vasopressor activity in the fractions obtained was tested in the isolated perfused rat kidney. 3. In one fraction a strong vasopressor action was found, which was blocked by saralasin and by an angiotensin II antibody. 4. Aprotinin inhibited the formation of vasopressor substances by tissue kallikrein. 5. U.v.-laser desorption/ionization mass spectrometry revealed a molecular mass of 1046 Da in the purified active fraction. 6. It is concluded that tissue kallikrein forms not only kinins, but also angiotensin II, from human plasma under physiological conditions.
KW - Angiotensin II
KW - Animals
KW - Aprotinin
KW - Blood Pressure
KW - Dose-Response Relationship, Drug
KW - Humans
KW - Kallikreins
KW - Kidney
KW - Mass Spectrometry
KW - Norepinephrine
KW - Rats
KW - Rats, Inbred WKY
KW - Saralasin
KW - Journal Article
M3 - SCORING: Journal article
C2 - 1385031
VL - 83
SP - 477
EP - 482
JO - CLIN SCI
JF - CLIN SCI
SN - 0143-5221
IS - 4
ER -