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Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types

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Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types. / Brancaccio, Rosario N; Robitaille, Alexis; Dutta, Sankhadeep; Cuenin, Cyrille; Santare, Daiga; Skenders, Girts; Leja, Marcis; Fischer, Nicole; Giuliano, Anna R; Rollison, Dana E; Grundhoff, Adam; Tommasino, Massimo; Gheit, Tarik.

In: VIROLOGY, Vol. 520, 07.2018, p. 1-10.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Brancaccio, RN, Robitaille, A, Dutta, S, Cuenin, C, Santare, D, Skenders, G, Leja, M, Fischer, N, Giuliano, AR, Rollison, DE, Grundhoff, A, Tommasino, M & Gheit, T 2018, 'Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types', VIROLOGY, vol. 520, pp. 1-10. https://doi.org/10.1016/j.virol.2018.04.017

APA

Brancaccio, R. N., Robitaille, A., Dutta, S., Cuenin, C., Santare, D., Skenders, G., Leja, M., Fischer, N., Giuliano, A. R., Rollison, D. E., Grundhoff, A., Tommasino, M., & Gheit, T. (2018). Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types. VIROLOGY, 520, 1-10. https://doi.org/10.1016/j.virol.2018.04.017

Vancouver

Brancaccio RN, Robitaille A, Dutta S, Cuenin C, Santare D, Skenders G et al. Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types. VIROLOGY. 2018 Jul;520:1-10. https://doi.org/10.1016/j.virol.2018.04.017

Bibtex

@article{7d524ca21f4f43c090a1375a06a28e86,
title = "Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types",
abstract = "With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.",
keywords = "Cohort Studies, DNA Primers, DNA, Viral, Genotype, High-Throughput Nucleotide Sequencing, Humans, Mouth, Papillomaviridae, Papillomavirus Infections, Polymerase Chain Reaction, Prospective Studies, Sequence Analysis, DNA, Skin, Journal Article, Research Support, Non-U.S. Gov't",
author = "Brancaccio, {Rosario N} and Alexis Robitaille and Sankhadeep Dutta and Cyrille Cuenin and Daiga Santare and Girts Skenders and Marcis Leja and Nicole Fischer and Giuliano, {Anna R} and Rollison, {Dana E} and Adam Grundhoff and Massimo Tommasino and Tarik Gheit",
note = "Copyright {\textcopyright} 2018. Published by Elsevier Inc.",
year = "2018",
month = jul,
doi = "10.1016/j.virol.2018.04.017",
language = "English",
volume = "520",
pages = "1--10",
journal = "VIROLOGY",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types

AU - Brancaccio, Rosario N

AU - Robitaille, Alexis

AU - Dutta, Sankhadeep

AU - Cuenin, Cyrille

AU - Santare, Daiga

AU - Skenders, Girts

AU - Leja, Marcis

AU - Fischer, Nicole

AU - Giuliano, Anna R

AU - Rollison, Dana E

AU - Grundhoff, Adam

AU - Tommasino, Massimo

AU - Gheit, Tarik

N1 - Copyright © 2018. Published by Elsevier Inc.

PY - 2018/7

Y1 - 2018/7

N2 - With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.

AB - With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.

KW - Cohort Studies

KW - DNA Primers

KW - DNA, Viral

KW - Genotype

KW - High-Throughput Nucleotide Sequencing

KW - Humans

KW - Mouth

KW - Papillomaviridae

KW - Papillomavirus Infections

KW - Polymerase Chain Reaction

KW - Prospective Studies

KW - Sequence Analysis, DNA

KW - Skin

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.virol.2018.04.017

DO - 10.1016/j.virol.2018.04.017

M3 - SCORING: Journal article

C2 - 29747121

VL - 520

SP - 1

EP - 10

JO - VIROLOGY

JF - VIROLOGY

SN - 0042-6822

ER -