Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types
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Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types. / Brancaccio, Rosario N; Robitaille, Alexis; Dutta, Sankhadeep; Cuenin, Cyrille; Santare, Daiga; Skenders, Girts; Leja, Marcis; Fischer, Nicole; Giuliano, Anna R; Rollison, Dana E; Grundhoff, Adam; Tommasino, Massimo; Gheit, Tarik.
in: VIROLOGY, Jahrgang 520, 07.2018, S. 1-10.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Generation of a novel next-generation sequencing-based method for the isolation of new human papillomavirus types
AU - Brancaccio, Rosario N
AU - Robitaille, Alexis
AU - Dutta, Sankhadeep
AU - Cuenin, Cyrille
AU - Santare, Daiga
AU - Skenders, Girts
AU - Leja, Marcis
AU - Fischer, Nicole
AU - Giuliano, Anna R
AU - Rollison, Dana E
AU - Grundhoff, Adam
AU - Tommasino, Massimo
AU - Gheit, Tarik
N1 - Copyright © 2018. Published by Elsevier Inc.
PY - 2018/7
Y1 - 2018/7
N2 - With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.
AB - With the advent of new molecular tools, the discovery of new papillomaviruses (PVs) has accelerated during the past decade, enabling the expansion of knowledge about the viral populations that inhabit the human body. Human PVs (HPVs) are etiologically linked to benign or malignant lesions of the skin and mucosa. The detection of HPV types can vary widely, depending mainly on the methodology and the quality of the biological sample. Next-generation sequencing is one of the most powerful tools, enabling the discovery of novel viruses in a wide range of biological material. Here, we report a novel protocol for the detection of known and unknown HPV types in human skin and oral gargle samples using improved PCR protocols combined with next-generation sequencing. We identified 105 putative new PV types in addition to 296 known types, thus providing important information about the viral distribution in the oral cavity and skin.
KW - Cohort Studies
KW - DNA Primers
KW - DNA, Viral
KW - Genotype
KW - High-Throughput Nucleotide Sequencing
KW - Humans
KW - Mouth
KW - Papillomaviridae
KW - Papillomavirus Infections
KW - Polymerase Chain Reaction
KW - Prospective Studies
KW - Sequence Analysis, DNA
KW - Skin
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.virol.2018.04.017
DO - 10.1016/j.virol.2018.04.017
M3 - SCORING: Journal article
C2 - 29747121
VL - 520
SP - 1
EP - 10
JO - VIROLOGY
JF - VIROLOGY
SN - 0042-6822
ER -