Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells
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Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells. / Pohlkamp, Theresa; Steller, Laura; May, Petra; Günther, Thomas; Schüle, Roland; Frotscher, Michael; Herz, Joachim; Bock, Hans H.
In: PLOS ONE, Vol. 9, No. 6, 01.01.2014, p. e100384.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells
AU - Pohlkamp, Theresa
AU - Steller, Laura
AU - May, Petra
AU - Günther, Thomas
AU - Schüle, Roland
AU - Frotscher, Michael
AU - Herz, Joachim
AU - Bock, Hans H
PY - 2014/1/1
Y1 - 2014/1/1
N2 - We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.
AB - We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.
KW - Animals
KW - Cerebellum
KW - Genetic Engineering
KW - Integrases
KW - Mice
KW - Mice, Transgenic
KW - Neurons
KW - Phosphopyruvate Hydratase
KW - Recombination, Genetic
U2 - 10.1371/journal.pone.0100384
DO - 10.1371/journal.pone.0100384
M3 - SCORING: Journal article
C2 - 24950299
VL - 9
SP - e100384
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 6
ER -