PortalPublikationenGeneration and characterization of an Nse-CreERT2 transgenic...

Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells

Standard

Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells. / Pohlkamp, Theresa; Steller, Laura; May, Petra; Günther, Thomas; Schüle, Roland; Frotscher, Michael; Herz, Joachim; Bock, Hans H.

in: PLOS ONE, Jahrgang 9, Nr. 6, 01.01.2014, S. e100384.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Pohlkamp, T, Steller, L, May, P, Günther, T, Schüle, R, Frotscher, M, Herz, J & Bock, HH 2014, 'Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells', PLOS ONE, Jg. 9, Nr. 6, S. e100384. https://doi.org/10.1371/journal.pone.0100384

APA

Pohlkamp, T., Steller, L., May, P., Günther, T., Schüle, R., Frotscher, M., Herz, J., & Bock, H. H. (2014). Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells. PLOS ONE, 9(6), e100384. https://doi.org/10.1371/journal.pone.0100384

Vancouver

Pohlkamp T, Steller L, May P, Günther T, Schüle R, Frotscher M et al. Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells. PLOS ONE. 2014 Jan 1;9(6):e100384. https://doi.org/10.1371/journal.pone.0100384

Bibtex

@article{a35a6904024e4f8c92d125f38afe7640,
title = "Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells",
abstract = "We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.",
keywords = "Animals, Cerebellum, Genetic Engineering, Integrases, Mice, Mice, Transgenic, Neurons, Phosphopyruvate Hydratase, Recombination, Genetic",
author = "Theresa Pohlkamp and Laura Steller and Petra May and Thomas G{\"u}nther and Roland Sch{\"u}le and Michael Frotscher and Joachim Herz and Bock, {Hans H}",
year = "2014",
month = jan,
day = "1",
doi = "10.1371/journal.pone.0100384",
language = "English",
volume = "9",
pages = "e100384",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells

AU - Pohlkamp, Theresa

AU - Steller, Laura

AU - May, Petra

AU - Günther, Thomas

AU - Schüle, Roland

AU - Frotscher, Michael

AU - Herz, Joachim

AU - Bock, Hans H

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.

AB - We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.

KW - Animals

KW - Cerebellum

KW - Genetic Engineering

KW - Integrases

KW - Mice

KW - Mice, Transgenic

KW - Neurons

KW - Phosphopyruvate Hydratase

KW - Recombination, Genetic

U2 - 10.1371/journal.pone.0100384

DO - 10.1371/journal.pone.0100384

M3 - SCORING: Journal article

C2 - 24950299

VL - 9

SP - e100384

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 6

ER -